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MIF Polyklonaler Antikörper

MIF Polyklonal Antikörper für WB, IF/ICC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus, Ratte

Anwendung

WB, IF/ICC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 30957-1-PBS

Synonyme

GIF

Filter:
  • Western Blot (WB) analysis of various lysates using MIF Polyclonal antibody (30957-1-AP)

    WB analysis using 30957-1-AP (same clone as 30957-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30957-1-AP (MIF antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30957-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Neuro-2a cells using MIF Polyclonal antibody (30957-1-AP)

    IF Staining of Neuro-2a using 30957-1-AP (same clone as 30957-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using MIF antibody (30957-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 30957-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using MIF Polyclonal antibody (30957-1-AP)

    IF Staining of NIH/3T3 using 30957-1-AP (same clone as 30957-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using MIF antibody (30957-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 30957-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of NIH/3T3 cells using MIF Polyclonal antibody (30957-1-AP)

    FC experiment of NIH/3T3 using 30957-1-AP (same clone as 30957-1-PBS)

    1X10^6 NIH/3T3 cells were intracellularly stained with 0.4 ug Anti-Mouse MIF (30957-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 30957-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

30957-1-PBS bindet in WB, IF/ICC, FC (Intra), Indirect ELISA MIF und zeigt Reaktivität mit Maus, Ratten

Getestete Reaktivität Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Rekombinantes Protein
Vollständiger Name macrophage migration inhibitory factor
Berechnetes Molekulargewicht13KD
Beobachtetes Molekulargewicht13 kDa
GenBank-ZugangsnummerNM_010798.2
Gene symbol Mif
Gene ID (NCBI) 17319
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MIF is a pleiotropic cytokine that contributes to the pathogenesis of many autoimmune diseases through its upstream immunoregulatory function and its polymorphic genetic locus. MIF is a highly conserved protein of 12.5 kDa, with evolutionarily ancient homologues in plants, protozoans, nematodes, and invertebrates.