MIF4GD Polyklonaler Antikörper

MIF4GD Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 24288-1-PBS

Synonyme

SLIP1, MIFD, MIF4G, hSLIP1, AD023

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  WB analysis using 24288-1-AP (same clone as 24288-1-PBS)

  mouse testis tissue was subjected to SDS PAGE followed by western blot with 24288-1-AP (MIF4GD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24288-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 24288-1-AP (same clone as 24288-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24288-1-AP (MIF4GD antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24288-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

24288-1-PBS bindet in WB, IHC, Indirect ELISA MIF4GD und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MIF4GD fusion protein Ag17931
• Vollständiger Name: MIF4G domain containing
• Berechnetes Molekulargewicht: 256 aa, 29 kDa
• Beobachtetes Molekulargewicht: ~25 kDa
• GenBank-Zugangsnummer: BC033759
• Gene symbol: MIF4GD
• Gene ID (NCBI): 57409
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MIF4GD (MIF4G domain containing), also known as MIFD, AD023, or SLIP1, is a 222 amino acid protein that contains one MIF4G domain. Localized to both the nucleus and the cytoplasm, MIF4GD plays a role in the replication-dependent translation of histone mRNAs, which differ from most eukaryotic mRNAs in that they end with a stem-loop instead of a poly-A tail. Specifically, MIF4GD interacts with SLBP, eIF4G and DAP-5. Via its interaction with SLBP, MIF4GD is thought to be tethered to the stem loops of histone mRNAs where it may facilitate the circularizing of the mRNAs, thereby enhancing their translation. Depletion of MIF4GD results in reduced histone translation and may lead to cell death, suggesting that MIF4GD plays an important role in cell survival. Two isoforms of MIF4GD exist due to alternative splicing events.