MIG6; ERRFI1 Rekombinanter Antikörper

MIG6; ERRFI1 Rekombinant Antikörper für WB, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

240447C3

Kat-Nr. : 83475-2-PBS

Synonyme

ERRFI1, 240447C3, GENE 33, MIG 6, MIG6

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  WB analysis using 83475-2-RR (same clone as 83475-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83475-2-RR (MIG6; ERRFI1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83475-2-PBS in a different storage buffer formulation.

• 

  FC experiment of HepG2 using 83475-2-RR (same clone as 83475-2-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug MIG6; ERRFI1 Recombinant antibody (83475-2-RR, Clone:240447C3) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83475-2-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP00464-3

  Cytometric bead array standard curve of MP00464-3, MIG6; ERRFI1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83475-1-PBS. Detection antibody: 83475-2-PBS. Standard: Ag33341. Range: 0.625-80 ng/mL.

• 

  Affinity and Kinetic Characterization of 83475-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83475-2-RR against Human MIG6; ERRFI1 were performed. The affinity constant is 2.04 nM.

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Geprüfte Anwendungen

Produktinformation

83475-2-PBS bindet in WB, Cytometric bead array, Indirect ELISA MIG6; ERRFI1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: MIG6; ERRFI1 fusion protein Ag33341
• Vollständiger Name: ERBB receptor feedback inhibitor 1
• Berechnetes Molekulargewicht: 51kd
• Beobachtetes Molekulargewicht: 51 kDa
• GenBank-Zugangsnummer: NM_018948
• Gene symbol: MIG6
• Gene ID (NCBI): 54206
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ERRFI1 (the product of mitogen-inducible gene 6, also known as MIG6) is an early response gene encoding a non-kinase scaffold adaptor protein induced by various stimuli such as hormones and stresses. ERRFI1 is considered a negative regulator of EGFR because it can directly bind to EGFR, inhibit the catalytic activity of EGFR, and mediate EGFR lysosomal degradation. ERRFI1 may play important roles in regulating stress response, maintaining homeostasis in tissues like joints or cardiac muscle, and functioning as a tumor suppressor.