MINK1 Polyklonaler Antikörper

MINK1 Polyklonal Antikörper für IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 13137-1-AP

Synonyme

B55, GCK family kinase MiNK, hMINK, hMINKbeta, MAP4K6, MEK kinase kinase 6, MEKKK 6, MINK, MINK1, Misshapen like kinase 1, Misshapen/NIK related kinase, YSK2, ZC3

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Galerie der Validierungsdaten

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  WB analysis using 13137-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13137-1-AP (MINK1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 13137-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13137-1-AP (MINK1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 13137-1-AP

  IP Result of anti-MINK1 (IP:13137-1-AP, 4ug; Detection:13137-1-AP 1:300) with mouse brain tissue lysate 4000ug.

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  IHC staining of human lymphoma using 13137-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 13137-1-AP (MINK1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lymphoma using 13137-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 13137-1-AP (MINK1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skeletal muscle using 13137-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 13137-1-AP (MINK1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skeletal muscle using 13137-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 13137-1-AP (MINK1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse brain using 13137-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13137-1-AP (MINK1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse brain using 13137-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13137-1-AP (MINK1 Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Lymphomgewebe, humanes Skelettmuskelgewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

13137-1-AP bindet in WB, IP, IHC, CoIP, ELISA MINK1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MINK1 fusion protein Ag3795
• Vollständiger Name: misshapen-like kinase 1 (zebrafish)
• Berechnetes Molekulargewicht: 1332 aa, 150 kDa
• Beobachtetes Molekulargewicht: 150 kDa
• GenBank-Zugangsnummer: BC034673
• Gene symbol: MINK1
• Gene ID (NCBI): 50488
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB,CoIP	Hum Mol Genet; Lung adenocarcinoma cell-derived exosomes promote M2 macrophage polarization through transmission of miR-3153 to activate the JNK signaling pathway; Authors - Lu Xu
rat	WB	Cardiovasc Ther; Effects of Vitamin D Deficiency on the Function of the Cardiac Autonomic Nervous System in Rats.; Authors - Xuemei Luo
mouse	IHC	Front Genet; MBD2 mediates Th17 cell differentiation by regulating MINK1 in Th17-dominant asthma; Authors - Zhifeng Chen