MIOS Polyklonaler Antikörper

MIOS Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 20826-1-AP

Synonyme

GATOR2 complex protein MIOS, Missing oocyte meiosis regulator homolog

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  WB analysis of K-562 using 20826-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 20826-1-AP (MIOS Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of rat liver using 20826-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 20826-1-AP (MIOS antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 20826-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20826-1-AP (MIOS Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 20826-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 20826-1-AP (MIOS Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 20826-1-AP

  IP result of anti-MIOS (IP:20826-1-AP, 4ug; Detection:20826-1-AP 1:300) with HeLa cells lysate 4000ug.

• 

  IHC staining of mouse skeletal muscle using 20826-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 20826-1-AP (MIOS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 20826-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 20826-1-AP (MIOS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 20826-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 20826-1-AP (MIOS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 20826-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 20826-1-AP (MIOS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, HeLa-Zellen, PC-3-Zellen, Rattenlebergewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: Maus-Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below

Produktinformation

20826-1-AP bindet in WB, IHC, IP, ELISA MIOS und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MIOS fusion protein Ag14802
• Vollständiger Name: missing oocyte, meiosis regulator, homolog (Drosophila)
• Berechnetes Molekulargewicht: 875 aa, 99 kDa
• Beobachtetes Molekulargewicht: 99 kDa
• GenBank-Zugangsnummer: BC005883
• Gene symbol: MIOS
• Gene ID (NCBI): 54468
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The mechanistic target of rapamycin complex 1 (mTORC1) controls growth by regulating anabolic and catabolic processes in response to environmental cues, including nutrients. Amino acids signal to mTORC1 through the Rag GTPases, which are regulated by several protein complexes, including GATOR1 and GATOR2. GATOR2, which has five components (WDR24, MIOS, WDR59, SEH1L, SEC13), is required for amino acids to activate mTORC1. PMID: 35831510

Publikationen

Species	Application	Title
pig	WB	Am J Physiol Endocrinol Metab; Intermittent bolus feeding does not enhance protein synthesis, myonuclear accretion, or lean growth more than continuous feeding in a premature piglet model.; Authors - Marko Rudar
human	WB	JCI Insight; GATOR2-dependent mTORC1 activity is a therapeutic vulnerability in FOXO1 fusion positive rhabdomyosarcoma; Authors - Jacqueline Morales ; KD Validated
human	WB	Nat Commun; Increased translation driven by non-canonical EZH2 creates a synthetic vulnerability in enzalutamide-resistant prostate cancer; Authors - Shankha S Chatterjee