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MIOS Rekombinanter Antikörper

MIOS Rekombinant Antikörper für IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

240832D12

Kat-Nr. : 83856-4-PBS

Synonyme

Missing oocyte meiosis regulator homolog, GATOR2 complex protein MIOS, 240832D12

Filter:
  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using MIOS Recombinant antibody (83856-4-RR)

    IF Staining of U2OS using 83856-4-RR (same clone as 83856-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using MIOS antibody (83856-4-RR, Clone: 240832D12 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83856-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83856-4-RR

    Affinity and Kinetic Characterization of 83856-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83856-4-RR against Human MIOS were performed. The affinity constant is 0.439 nM.

Geprüfte Anwendungen

Produktinformation

83856-4-PBS bindet in IF/ICC, Indirect ELISA MIOS und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen MIOS fusion protein Ag15148
Vollständiger Name missing oocyte, meiosis regulator, homolog (Drosophila)
Berechnetes Molekulargewicht 875 aa, 99 kDa
GenBank-ZugangsnummerBC005883
Gene symbol MIOS
Gene ID (NCBI) 54468
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein A purfication
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The mechanistic target of rapamycin complex 1 (mTORC1) controls growth by regulating anabolic and catabolic processes in response to environmental cues, including nutrients. Amino acids signal to mTORC1 through the Rag GTPases, which are regulated by several protein complexes, including GATOR1 and GATOR2. GATOR2, which has five components (WDR24, MIOS, WDR59, SEH1L, SEC13), is required for amino acids to activate mTORC1. PMID: 3583151