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MIOS Rekombinanter Antikörper

MIOS Rekombinant Antikörper für IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

240832D12

Kat-Nr. : 83856-4-RR

Synonyme

Missing oocyte meiosis regulator homolog, GATOR2 complex protein MIOS, 240832D12

Filter:
  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using MIOS Recombinant antibody (83856-4-RR)

    IF Staining of U2OS using 83856-4-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using MIOS antibody (83856-4-RR, Clone: 240832D12 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 83856-4-RR

    Affinity and Kinetic Characterization of 83856-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83856-4-RR against Human MIOS were performed. The affinity constant is 0.439 nM.

Geprüfte Anwendungen

Erfolgreiche Detektion in IF/ICCU2OS-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

83856-4-RR bindet in IF/ICC, ELISA MIOS und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen MIOS fusion protein Ag15148
Vollständiger Name missing oocyte, meiosis regulator, homolog (Drosophila)
Berechnetes Molekulargewicht 875 aa, 99 kDa
GenBank-ZugangsnummerBC005883
Gene symbol MIOS
Gene ID (NCBI) 54468
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein A purfication
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The mechanistic target of rapamycin complex 1 (mTORC1) controls growth by regulating anabolic and catabolic processes in response to environmental cues, including nutrients. Amino acids signal to mTORC1 through the Rag GTPases, which are regulated by several protein complexes, including GATOR1 and GATOR2. GATOR2, which has five components (WDR24, MIOS, WDR59, SEH1L, SEC13), is required for amino acids to activate mTORC1. PMID: 3583151

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
IF protocol for MIOS antibody 83856-4-RRProtokoll herunterladen
STANDARD-PROTOKOLLE
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