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MIOS Rekombinanter Antikörper

MIOS Rekombinant Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

240832B9

Kat-Nr. : 83856-5-PBS

Synonyme

240832B9, GATOR2 complex protein MIOS, Missing oocyte meiosis regulator homolog

Filter:
  • Western Blot (WB) analysis of various lysates using MIOS Recombinant monoclonal antibody (83856-5-RR)

    WB analysis using 83856-5-RR (same clone as 83856-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83856-5-RR (MIOS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83856-5-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using MIOS Recombinant monoclonal antibody (83856-5-RR)

    WB analysis using 83856-5-RR (same clone as 83856-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83856-5-RR (MIOS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83856-5-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using MIOS Recombinant monoclonal antibody (83856-5-RR)

    WB analysis of HeLa using 83856-5-RR (same clone as 83856-5-PBS)

    WB result of MIOS antibody (83856-5-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MIOS transfected HeLa cells. This data was developed using the same antibody clone with 83856-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83856-5-RR

    Affinity and Kinetic Characterization of 83856-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83856-5-RR against Human MIOS were performed. The affinity constant is below 1 pM.

Geprüfte Anwendungen

Produktinformation

83856-5-PBS bindet in WB, Indirect ELISA MIOS und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen MIOS fusion protein Ag15148
Vollständiger Name missing oocyte, meiosis regulator, homolog (Drosophila)
Berechnetes Molekulargewicht 875 aa, 99 kDa
Beobachtetes Molekulargewicht 82 kDa
GenBank-ZugangsnummerBC005883
Gene symbol MIOS
Gene ID (NCBI) 54468
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein A purfication
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The mechanistic target of rapamycin complex 1 (mTORC1) controls growth by regulating anabolic and catabolic processes in response to environmental cues, including nutrients. Amino acids signal to mTORC1 through the Rag GTPases, which are regulated by several protein complexes, including GATOR1 and GATOR2. GATOR2, which has five components (WDR24, MIOS, WDR59, SEH1L, SEC13), is required for amino acids to activate mTORC1. PMID: 35831510