CCL19/MIP-3 beta Polyklonaler Antikörper

CCL19/MIP-3 beta Polyklonal Antikörper für WB, IF/ICC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13397-1-PBS

Synonyme

CCL19, MIP-3 Beta, MIP-3-beta, Beta chemokine exodus 3, Beta-chemokine exodus-3

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  WB analysis using 13397-1-AP (same clone as 13397-1-PBS)

  THP-1 cells were subjected to SDS PAGE followed by western blot with 13397-1-AP (MIP-3 Beta antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13397-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 13397-1-AP (same clone as 13397-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using MIP-3 Beta antibody (13397-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 13397-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 13397-1-AP (same clone as 13397-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol) fixed A549 cells using 13397-1-AP (MIP-3-beta antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13397-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 13397-1-AP (same clone as 13397-1-PBS)

  Immunofluorescent analysis of A549 cells using 13397-1-AP (MIP-3-beta antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13397-1-PBS in a different storage buffer formulation.

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  FC experiment of A549 using 13397-1-AP (same clone as 13397-1-PBS)

  1X10^6 A549 cells were intracellularly stained with 0.4 ug Anti-Human MIP-3 Beta (13397-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 13397-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

13397-1-PBS bindet in WB, IF/ICC, FC (Intra), Indirect ELISA CCL19/MIP-3 beta und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CCL19/MIP-3 beta fusion protein Ag4200
• Vollständiger Name: chemokine (C-C motif) ligand 19
• Berechnetes Molekulargewicht: 98 aa, 12 kDa
• Beobachtetes Molekulargewicht: 11 kDa
• GenBank-Zugangsnummer: BC027968
• Gene symbol: CCL19/MIP-3 beta
• Gene ID (NCBI): 6363
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CCL19, also known as MIP-3β, is one of several CC cytokine genes clustered on the p-arm of chromosome 9. CCL19 may play a role in normal lymphocyte recirculation and homing. It also plays an important role in trafficking of T cells in thymus, and in T cell and B cell migration to secondary lymphoid organs. It specifically binds to chemokine receptor CCR7.