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MIS12 Polyklonaler Antikörper

MIS12 Polyklonal Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 19820-1-PBS

Synonyme

Filter:
  • Western Blot (WB) analysis of various lysates using MIS12 Polyclonal antibody (19820-1-AP)

    WB analysis using 19820-1-AP (same clone as 19820-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 19820-1-AP (MIS12 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19820-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

19820-1-PBS bindet in WB, Indirect ELISA MIS12 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen MIS12 fusion protein Ag13870
Vollständiger Name MIS12, MIND kinetochore complex component, homolog (yeast)
Berechnetes Molekulargewicht 205 aa, 24 kDa
Beobachtetes Molekulargewicht 25-28 kDa
GenBank-ZugangsnummerBC000229
Gene symbol MIS12
Gene ID (NCBI) 79003
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The MIS12 complex, composed of the Mis12, Pmf1, Nsl1 and Dsn1 subunits, is a highly conserved kinetochore protein that interacts with the Ndc80 and Knl1 complexes, forming the KMN network (PMID: 32341029). MIS12 is a part of the MIS12 complex which is required for normal chromosome alignment and segregation and for kinetochore formation during mitosis. MIS12 is essential for proper kinetochore microtubule attachments (PubMed:23891108)