MKNK2 Polyklonaler Antikörper

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• KD/KO Validated

MKNK2 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 17354-1-AP

Synonyme

MNK2, MAPK signal-integrating kinase 2, MAP kinase-interacting serine/threonine-protein kinase 2, MAP kinase signal-integrating kinase 2, GPRK7

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  WB analysis of mouse spleen using 17354-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 17354-1-AP (MKNK2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 17354-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 17354-1-AP (MKNK2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 17354-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17354-1-AP (MKNK2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 17354-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17354-1-AP (MKNK2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 17354-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using MKNK2 antibody (17354-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausmilzgewebe, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:100-1:400
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 1 publications below

Produktinformation

17354-1-AP bindet in WB, IHC, IF/ICC, ELISA MKNK2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MKNK2 fusion protein Ag10546
• Vollständiger Name: MAP kinase interacting serine/threonine kinase 2
• Berechnetes Molekulargewicht: 465 aa, 52 kDa
• Beobachtetes Molekulargewicht: 47-52 kDa
• GenBank-Zugangsnummer: BC018345
• Gene symbol: MKNK2
• Gene ID (NCBI): 2872
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MKNK2(MAP kinase-interacting serine/threonine-protein kinase 2) is also named as GPRK7, MNK2 and belongs to the CAMK Ser/Thr protein kinase family. It has a conserved C-terminal Erk-interacting domain, a catalytic domain with homology to the calcium/calmodulin-dependent family of kinases, and putative MAP kinase phosphorylation sites located within the T loop of the kinase domain(PMID:9155017). The breast cancer cells express either an uncharacterized form of MNK2 or a cross-reacting MNK2-like protein with the band of 72-75 kDa by western blot(PMID:17130135). MKNK2 has 2 isoforms with the molecular mass of 52 kDa and 47 kDa.

Publikationen

Species	Application	Title
human		Clin Sci (Lond); Down-regulation of the insulin signaling pathway by SHC may correlate with congenital heart disease in Chinese populations.; Authors - Zhiling Luo
human	WB	Oncotarget; Impact of eIF4E phosphorylation at Ser209 via MNK2a on tumour recurrence after curative surgery in localized clear cell renal cell carcinoma.; Authors - Osamu Ichiyanagi
human	WB	Oncotarget; Inhibitory effects of Tomivosertib in acute myeloid leukemia.; Authors - Milagros Suarez
human,mouse	WB,IHC	Inflammation; High Expression of miR-6785-5p in the Serum Exosomes of Psoriasis Patients Alleviates Psoriasis-Like Skin Damage by Interfering with the MNK2/p-eIF4E Axis in Keratinocytes; Authors - Ruijie Wang ; KD Validated
mouse	WB	J Agric Food Chem; Non-Sugar Sweetener Rubusoside Alleviates Lipid Metabolism Disorder In Vivo and In Vitro by Targeting PPARγ/α, Lgals3, and Mknk2; Authors - Wanfang Huang