MKRN2 Polyklonaler Antikörper

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• KD/KO Validated

MKRN2 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12238-1-PBS

Synonyme

E3 ubiquitin-protein ligase makorin-2, EC:2.3.2.27, HSPC070, makorin ring finger protein 2, RING finger protein 62

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  WB analysis using 12238-1-AP (same clone as 12238-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12238-1-AP (MKRN2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12238-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 12238-1-AP (same clone as 12238-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 12238-1-AP (MKRN2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12238-1-PBS in a different storage buffer formulation.

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  WB analysis of A375 using 12238-1-AP (same clone as 12238-1-PBS)

  A375 cells were subjected to SDS PAGE followed by western blot with 12238-1-AP (MKRN2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12238-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 12238-1-AP (same clone as 12238-1-PBS)

  IP result of anti-MKRN2 (IP:12238-1-AP, 4ug; Detection:12238-1-AP 1:2000) with HeLa cells lysate 1400 ug. This data was developed using the same antibody clone with 12238-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 12238-1-AP (same clone as 12238-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12238-1-AP (MKRN2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12238-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 12238-1-AP (same clone as 12238-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12238-1-AP (MKRN2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12238-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 12238-1-AP (same clone as 12238-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using MKRN2 antibody (12238-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12238-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 12238-1-AP (same clone as 12238-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using MKRN2 antibody (12238-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12238-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

12238-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA MKRN2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MKRN2 fusion protein Ag2881
• Vollständiger Name: makorin ring finger protein 2
• Berechnetes Molekulargewicht: 416 aa, 47 kDa
• Beobachtetes Molekulargewicht: 47 kDa
• GenBank-Zugangsnummer: BC015715
• Gene symbol: MKRN2
• Gene ID (NCBI): 23609
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MKRN2(Probable E3 ubiquitin-protein ligase makorin-2) is also named as RNF62. It is an E3 ubiquitin ligase that catalyzes the covalent attachment of ubiquitin moieties onto substrate proteins. It may be playing a functional role in negatively regulating neurogenesis via PI3K/Akt signaling.