Melan-A Monoklonaler Antikörper

Melan-A Monoklonal Antikörper für IHC, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

CloneNo.

4F10D6

Kat-Nr. : 60348-1-Ig

Synonyme

Antigen LB39 AA, Antigen SK29 AA, MART 1, MART1, melan A, Melan-A , MLANA, Protein Melan A

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  IHC staining of human malignant melanoma using 60348-1-Ig

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 60348-1-Ig (Melan-A antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human malignant melanoma using 60348-1-Ig

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 60348-1-Ig (Melan-A antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung using 60348-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 60348-1-Ig (Melan-A antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung using 60348-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 60348-1-Ig (Melan-A antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human malignant melanoma using 60348-1-Ig

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 60348-1-Ig (MLANA Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human malignant melanoma using 60348-1-Ig

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 60348-1-Ig (MLANA Antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes malignes Melanomgewebe, humanes Lungengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:2000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 3 publications below

Produktinformation

60348-1-Ig bindet in IHC, IF, ELISA Melan-A und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Melan-A fusion protein Ag13387
• Vollständiger Name: melan-A
• Berechnetes Molekulargewicht: 13 kDa
• GenBank-Zugangsnummer: BC014423
• Gene symbol: MelanA
• Gene ID (NCBI): 2315
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Melan-A is a melanocyte differentiation antigen, recognized by autologous cytotoxic T lymphocytes. Melan-A is also called MART-1 (melanoma antigen recognized by T cells). The Melan-A/MART-1 gene encodes this protein, 20-22 kDa, associated with endoplasmic reticulum and melanosomes. The function of the protein is unknown. Melan-A, isolated as a melanoma-specific antigen, is a transmembrane protein, which is expressed in skin, retina and the majority of cultured melanocytes as well as in melanomas. Melan A is expressed in more than 85% of melanomas.

Publikationen

Species	Application	Title
human	IF	Cancer Commun (Lond); Candidate therapeutic agents in a newly established triple wild-type mucosal melanoma cell line.; Authors - Chaoji Shi
human	IF	Invest Ophthalmol Vis Sci; The Spatial Transcriptomic Atlas of Human Limbus and Vital Niche Microenvironment Regulating the Fate of Limbal Epithelial Stem Cells; Authors - Shiding Li
human	IF	Inflammation; Sirtuin1 Deficiency Could Exacerbate Melanocyte Apoptosis Under Endoplasmic Reticulum Stress; Authors - Jing Zhu