MLF1IP Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

MLF1IP Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13186-1-PBS

Synonyme

CENP 50, CENP U, CENP U(50), CENP50, CENP-50

---

• 

  WB analysis using 13186-1-AP (same clone as 13186-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13186-1-AP (MLF1IP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13186-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 13186-1-AP (same clone as 13186-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13186-1-AP (MLF1IP antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13186-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse colon using 13186-1-AP (same clone as 13186-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 13186-1-AP (MLF1IP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13186-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse colon using 13186-1-AP (same clone as 13186-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 13186-1-AP (MLF1IP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13186-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 13186-1-AP (same clone as 13186-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13186-1-AP (MLF1IP Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 13186-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 13186-1-AP (same clone as 13186-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13186-1-AP (MLF1IP Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 13186-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 13186-1-AP (same clone as 13186-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13186-1-AP (MLF1IP Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 13186-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 13186-1-AP (same clone as 13186-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13186-1-AP (MLF1IP Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 13186-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 13186-1-AP (same clone as 13186-1-PBS)

  Immunofluorescent analysis of HepG2 cells using 13186-1-AP (MLF1IP antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13186-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

13186-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA MLF1IP und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MLF1IP fusion protein Ag3858
• Vollständiger Name: MLF1 interacting protein
• Berechnetes Molekulargewicht: 418 aa, 48 kDa
• Beobachtetes Molekulargewicht: 46-50 kDa, 60 kDa
• GenBank-Zugangsnummer: BC031520
• Gene symbol: MLF1IP
• Gene ID (NCBI): 79682
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MLF1IP, also named as CENPU, ICEN24, KLIP1 and PBIP1, belongs to the CENP-U/AME1 family. MLF1IP plays an important role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. Moreover, MLF1IP has been shown to be involved in tumorigenesis in several types of cancer including glioblastoma, prostate cancer and luminal breast cancer (PMID: 27378428). MLF1IP has 3 isoforms with the molecular mass of 21, 38 and 47 kDa.