MLH1 Monoklonaler Antikörper

MLH1 Monoklonal Antikörper für IHC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

2D5A2

Kat-Nr. : 60822-2-Ig

Synonyme

2D5A2, COCA2, FCC2, hMLH1, HNPCC

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  IHC staining of human appendicitis using 60822-2-Ig

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60822-2-Ig (MLH1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human rectal cancer using 60822-2-Ig

  Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 60822-2-Ig (MLH1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 60822-2-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60822-2-Ig (MLH1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 60822-2-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60822-2-Ig (MLH1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human appendicitis using 60822-2-Ig

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60822-2-Ig (MLH1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human rectal cancer using 60822-2-Ig

  Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 60822-2-Ig (MLH1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of Human tonsillitis using 60822-2-Ig

  Immunohistochemical analysis of paraffin-embedded Human tonsillitis tissue slide using 60822-2-Ig (MLH1 antibody) at dilution of 1:2000 (under 10x lens).

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  IHC staining of Human tonsillitis using 60822-2-Ig

  Immunohistochemical analysis of paraffin-embedded Human tonsillitis tissue slide using 60822-2-Ig (MLH1 antibody) at dilution of 1:2000 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, humanes Appendizitis-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

60822-2-Ig bindet in IHC, ELISA MLH1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: MLH1 fusion protein Ag27723
• Vollständiger Name: mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli)
• Berechnetes Molekulargewicht: 756 aa, 85 kDa
• GenBank-Zugangsnummer: BC006850
• Gene symbol: MLH1
• Gene ID (NCBI): 4292
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MLH1, also named as COCA2, belongs to the DNA mismatch repair mutL/hexB family. It heterodimerizes with PMS2 to form MutL alpha which is a component of the post-replicative DNA mismatch repair system (MMR). MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. MLH1 also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. MLH1 heterodimerizes with MLH3 to form MutL gamma which plays a role in meiosis.(PMID: 16873062, PMID: 18206974) Defects in MLH1 are the cause of hereditary non-polyposis colorectal cancer type 2 (HNPCC2). Defects in MLH1 are a cause of mismatch repair cancer syndrome (MMRCS). Defects in MLH1 are a cause of Muir-Torre syndrome (MTS). Defects in MLH1 are a cause of susceptibility to endometrial cancer.