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MLH1 Rekombinanter Antikörper

MLH1 Rekombinant Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241472D1

Kat-Nr. : 84208-2-PBS

Synonyme

241472D1, COCA2, FCC2, hMLH1, HNPCC

Filter:
  • Western Blot (WB) analysis of various lysates using MLH1 Recombinant antibody (84208-2-RR)

    WB analysis using 84208-2-RR (same clone as 84208-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84208-2-RR (MLH1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84208-2-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using MLH1 Recombinant antibody (84208-2-RR)

    IP experiment of HeLa using 84208-2-RR (same clone as 84208-2-PBS)

    IP result of anti-MLH1 (IP:84208-2-RR, 4ug; Detection:84208-2-RR 1:1000) with HeLa cells lysate 1200 ug. This data was developed using the same antibody clone with 84208-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using MLH1 Recombinant antibody (84208-2-RR)

    IHC staining of human colon cancer using 84208-2-RR (same clone as 84208-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84208-2-RR (MLH1 antibody) at dilution of 1:250 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84208-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using MLH1 Recombinant antibody (84208-2-RR)

    IF Staining of HeLa using 84208-2-RR (same clone as 84208-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MLH1 antibody (84208-2-RR, Clone: 241472D1 ) at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84208-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using MLH1 Recombinant antibody (84208-2-RR)

    IF Staining of HeLa using 84208-2-RR (same clone as 84208-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MLH1 antibody (84208-2-RR, Clone: 241472D1 ) at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 84208-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84208-2-PBS

    Affinity and Kinetic Characterization of 84208-2-PBS

    Biolayer interferometry (BLl) kinetic assay of 84208-2-PBS against Human MLH1 was performed. The affinity constant is below 1 pM.

  • Affinity and Kinetic Characterization of 84208-2-RR

    Affinity and Kinetic Characterization of 84208-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84208-2-RR against Human MLH1 were performed. The affinity constant is below 1 pM.

Geprüfte Anwendungen

Produktinformation

84208-2-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA MLH1 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen MLH1 fusion protein Ag2319
Vollständiger Name mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli)
Berechnetes Molekulargewicht 756 aa, 85 kDa
Beobachtetes Molekulargewicht85 kDa
GenBank-ZugangsnummerBC006850
Gene symbol MLH1
Gene ID (NCBI) 4292
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MLH1, also named as COCA2, belongs to the DNA mismatch repair mutL/hexB family. It heterodimerizes with PMS2 to form MutL alpha which is a component of the post-replicative DNA mismatch repair system (MMR). MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. MLH1 also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. MLH1 heterodimerizes with MLH3 to form MutL gamma which plays a role in meiosis.(PMID: 16873062, PMID: 18206974) Defects in MLH1 are the cause of hereditary non-polyposis colorectal cancer type 2 (HNPCC2). Defects in MLH1 are a cause of mismatch repair cancer syndrome (MMRCS). Defects in MLH1 are a cause of Muir-Torre syndrome (MTS). Defects in MLH1 are a cause of susceptibility to endometrial cancer. Western blot analysis with an MLH1 antibody detected a 85-100 kDa band.