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MLK4 Polyklonaler Antikörper

MLK4 Polyklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 24395-1-PBS

Synonyme

Filter:
  • Western Blot (WB) analysis of HeLa cells using MLK4 Polyclonal antibody (24395-1-AP)

    WB analysis of HeLa using 24395-1-AP (same clone as 24395-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 24395-1-AP (MLK4 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24395-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of C6 cells using MLK4 Polyclonal antibody (24395-1-AP)

    IF Staining of C6 using 24395-1-AP (same clone as 24395-1-PBS)

    Immunofluorescent analysis of C6 cells using 24395-1-AP (MLK4 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG. This data was developed using the same antibody clone with 24395-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

24395-1-PBS bindet in WB, IF/ICC, Indirect ELISA MLK4 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen MLK4 fusion protein Ag18904
Vollständiger Name mixed lineage kinase 4
Berechnetes Molekulargewicht 1036 aa, 114 kDa
Beobachtetes Molekulargewicht 53 kDa
GenBank-ZugangsnummerBC136649
Gene symbol MLK4
Gene ID (NCBI) 84451
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MLK4 belongs to the MAP kinase kinase kinase subfamily. The MLK family members are characterized by the presence of signature sequences ofSer/Thr and Tyr kinases within their catalytic domain. The MLK1-4 contains an aminoterminal Src-Homology (SH3) domain, followed by the kinase domain, a leucine-zipper region, and a Cdc42/Rac-interactive binding (CRIB) motif. MLK4 expressed in two splicing variants, MLK4α and MLK4β. The difference of these two isoforms is located in their C-terminal portion. The negative effect of MLK4 on LPS-induced ERK and JNK activation could be very important in controlling the level of cytokine expression, and thus maintaining the balance of inflammatory responses.(PMID:21602844). This protein has 3 isoforms produced by alternative splicing with the molecular weight of 63 kDa, 113 kDa, 53 kDa.