MLKL Monoklonaler Antikörper

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• KD/KO Validated

MLKL Monoklonal Antikörper für IF/ICC, IF-P

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

IF/ICC, IF-P

Konjugation

CoraLite® Plus 488 Fluorescent Dye

Publikationen(2)

CloneNo.

3D4C6

Kat-Nr. : CL488-66675

Synonyme

Mixed lineage kinase domain-like protein, hMLKL, 3D4C6

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  IF Staining of human liver cancer using CL488-66675

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite® Plus 488-conjugated MLKL antibody (CL488-66675, Clone: 3D4C6 ) at dilution of 1:100.

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  IF Staining of human liver cancer using CL488-66675

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CL488-66675 (MLKL antibody) at dilution of 1:50.

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  IF Staining of HepG2 using CL488-66675

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 MLKL antibody (CL488-66675, Clone: 3D4C6 ) at dilution of 1:200.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF-P: humanes Leberkarzinomgewebe
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 2 publications below
• FC: See 1 publications below

Produktinformation

CL488-66675 bindet in IF/ICC, IF-P MLKL und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: MLKL fusion protein Ag15188
• Vollständiger Name: mixed lineage kinase domain-like
• Berechnetes Molekulargewicht: 471 aa, 54 kDa
• Beobachtetes Molekulargewicht: 54 kDa
• GenBank-Zugangsnummer: BC028141
• Gene symbol: MLKL
• Gene ID (NCBI): 197259
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Mixed lineage kinase domain like pseudokinase (MLKL), belongs to the protein kinase superfamily, has two MW of 54 and 30 kDa. MLKL plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children.

Publikationen

Species	Application	Title
mouse	IF,FC	J Transl Med; TREM-1 triggers necroptosis of macrophages through mTOR-dependent mitochondrial fission during acute lung injury; Authors - Wen-Jing Zhong
	IF	Int J Biol Sci; Neutrophil extracellular traps trigger alveolar epithelial cell necroptosis through the cGAS-STING pathway during acute lung injury in mice; Authors - Han-Xi Sha