KMT2A Polyklonaler Antikörper

KMT2A Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 29278-1-AP

Synonyme

MLL, HTRX, HRX, FLJ11783, CXXC7

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  WB analysis using 29278-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29278-1-AP (MLL antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, K-562-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IF: See 1 publications below
• ChIP: See 1 publications below

Produktinformation

29278-1-AP bindet in WB, IF, ChIP, ELISA KMT2A und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: KMT2A fusion protein Ag30468
• Vollständiger Name: myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila)
• Berechnetes Molekulargewicht: 432 kDa
• Beobachtetes Molekulargewicht: 180 kDa
• GenBank-Zugangsnummer: NM_001197104
• Gene symbol: MLL
• Gene ID (NCBI): 4297
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KMT2A, also known as mixed-lineage leukemia (MLL), is a gene that encodes a lysine methyltransferase responsible for the transcriptional activation through lysine 4 of histone 3 (H3K4) methylation. KMT2A is expressed mainly in the nucleus and is ubiquitously present in various tissues, particularly in ovary, lymph node, endometrium, thyroid, and brain tissue. It plays a significant role in embryonic development, hematopoiesis, and neurodevelopment. Mutations in KMT2A have been associated with several pathological conditions.

Publikationen

Species	Application	Title
human	WB,ChIP	Nat Commun; KMT2A associates with PHF5A-PHF14-HMG20A-RAI1 subcomplex in pancreatic cancer stem cells and epigenetically regulates their characteristics; Authors - Mai Abdel Mouti
human	IF	J Inflamm (Lond); Histone H3 posttranslational modified enzymes defined neutrophil plasticity and their vulnerability to IL-10 in the course of the inflammation; Authors - Paweł Piatek
human	WB	Cancer Lett; PARP1 interacts with WDR5 to enhance target gene recognition and facilitate tumorigenesis; Authors - Yali Qin