MLL4 Rekombinanter Antikörper

MLL4 Rekombinant Antikörper für WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte

Anwendung

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

241819A4

Kat-Nr. : 84410-1-PBS

Synonyme

241819A4, CXXC10 , DYT28, HRX2, KIAA0304

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  WB analysis using 84410-1-RR (same clone as 84410-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84410-1-RR (MLL4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84410-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP01298-1

  Sandwich ELISA standard curve of MP01298-1, Human MLL4 Recombinant Matched Antibody Pair - PBS only. 84410-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag35385. 84410-2-PBS was HRP conjugated as the detection antibody. Range: 46.9-3000 pg/mL.

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  Sample test of MP01298-1

  The mean MLL4 concentration was determined to be 1.5 ng/mL in HeLa cell extract based on a 1.8 mg/mL extract load.

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  Cytometric bead array standard curve of MP01298-1

  Cytometric bead array standard curve of MP01298-1, MLL4 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84410-1-PBS. Detection antibody: 84410-2-PBS. Standard: Ag35385. Range: 0.781-100 ng/mL.

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  Affinity and Kinetic Characterization of 84410-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84410-1-RR against Human MLL4 were performed. The affinity constant is 22.6 pM.

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Geprüfte Anwendungen

Produktinformation

84410-1-PBS bindet in WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test MLL4 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: MLL4 fusion protein Ag35385
• Vollständiger Name: myeloid/lymphoid or mixed-lineage leukemia 4
• Beobachtetes Molekulargewicht: 294 kDa
• GenBank-Zugangsnummer: BC009337
• Gene symbol: MLL4
• Gene ID (NCBI): 9757
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MLL4, also known as KMT2B, which is an essential subunit of the H3K4 methylation complexes, regulates cell type-specific transcriptional programs through enhancer activation. Taspase 1 mediates cleavage of the MLL protein, generating cleavage products.