MLLT1 Rekombinanter Antikörper

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• KD/KO Validated

MLLT1 Rekombinant Antikörper für WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

230519E7

Kat-Nr. : 83242-3-PBS

Synonyme

230519E7, ENL, LTG19, MLLT 1, Protein ENL

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  WB analysis using 83242-3-RR (same clone as 83242-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83242-3-RR (MLLT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83242-3-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 83242-3-RR (same clone as 83242-3-PBS)

  WB result of MLLT1 antibody (83242-3-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MLLT1 transfected HeLa cells. This data was developed using the same antibody clone with 83242-3-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP00158-2

  Sandwich ELISA standard curve of MP00158-2, Human MLLT1 Recombinant Matched Antibody Pair - PBS only. 83242-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag34210. 83242-4-PBS was HRP conjugated as the detection antibody. Range: 31.3-2000 pg/mL

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  Sample test of MP00158-2

  Hela and U-251 cell lysates were measured. The human MLLT1 concentration of detected samples was determined to be 16384.9 pg/mL (based on a 6.4 mg/mL extract load) in Hela cell lysate and 14242.8 pg/mL (based on a 6.4 mg/mL extract load) in U-251 cell lysate.

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  IF Staining of HeLa using 83242-3-RR (same clone as 83242-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MLLT1 antibody (83242-3-RR, Clone: 230519E7 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83242-3-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 83242-3-RR (same clone as 83242-3-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug MLLT1 Recombinant antibody (83242-3-RR, Clone:230519E7) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83242-3-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00158-2

  Cytometric bead array standard curve of MP00158-2, MLLT1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83242-3-PBS. Detection antibody: 83242-4-PBS. Standard: Ag34210. Range: 1.25-40 ng/mL.

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  Affinity and Kinetic Characterization of 83242-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83242-3-RR against Human MLLT1 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

83242-3-PBS bindet in WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test MLLT1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: MLLT1 fusion protein Ag34210
• Vollständiger Name: myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 1
• Berechnetes Molekulargewicht: 62 kDa
• Beobachtetes Molekulargewicht: 75 kDa
• GenBank-Zugangsnummer: BC148806
• Gene symbol: MLLT1
• Gene ID (NCBI): 4298
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.