MLN64 Polyklonaler Antikörper

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• KD/KO Validated

MLN64 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 20292-1-AP

Synonyme

CAB1, MLN 64, MLN64, Protein CAB1, STARD3

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  WB analysis of Jurkat using 20292-1-AP

  WB result of MLN64 antibody (20292-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MLN64 transfected Jurkat cells.

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  WB analysis of MCF-7 using 20292-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 20292-1-AP (MLN64 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 20292-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20292-1-AP (MLN64 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse placenta using 20292-1-AP

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 20292-1-AP (MLN64 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of MCF-7 using 20292-1-AP

  IP result of anti-MLN64 (IP:20292-1-AP, 3ug; Detection:20292-1-AP 1:500) with MCF-7 cells lysate 1200ug.

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  IHC staining of human ovary tumor using 20292-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 20292-1-AP (MLN64 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human ovary tumor using 20292-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 20292-1-AP (MLN64 antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of MCF-7 using 20292-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 20292-1-AP (MLN64 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF7 cells, A549-Zellen, Jurkat-Zellen, MCF-7-Zellen, Maushirngewebe, Maus-Plazenta-Gewebe, Rattenhirngewebe
• Erfolgreiche IP: MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

20292-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA MLN64 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MLN64 fusion protein Ag14061
• Vollständiger Name: StAR-related lipid transfer (START) domain containing 3
• Berechnetes Molekulargewicht: 445 aa, 51 kDa
• Beobachtetes Molekulargewicht: 33-53 kDa
• GenBank-Zugangsnummer: BC008747
• Gene symbol: MLN64/STARD3
• Gene ID (NCBI): 10948
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MLN64 (also known as STARD3) is an integral membrane protein anchored in late endosomes. MLN64 has a StAR-related lipid-transfer (START) domain to bind cholesterol, mediating intracellular cholesterol transfer. MLN64 is widely expressed in multiple tissues including liver, spleen, heart, kidney, lung, and the brain. Higher expression of MLN64 has been found in several malignancies. MLN64 can be detected as 33 kDa truncated fragment (PMID:9237999, PMID:15718238).

Publikationen

Species	Application	Title
human	WB	Autophagy; PMID: 24905460; Authors - Rui Liu
human,mouse	WB,IHC,IF,IP	Life Sci; Blockade of STARD3-mediated cholesterol transport alleviates diabetes-induced podocyte injury by reducing mitochondrial cholesterol accumulation; Authors - Jijia Hu ; KD Validated
human	WB	J Cell Sci; The lysosomal lipid transporter LIMP-2 is part of lysosome-ER STARD3-VAPB-dependent contact sites; Authors - Sönke Rudnik