MLPH Polyklonaler Antikörper

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MLPH Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10338-1-PBS

Synonyme

Exophilin 3, Exophilin-3, Melanophilin, SLAC2 A, SLAC2A

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  WB analysis using 10338-1-AP (same clone as 10338-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10338-1-AP (MLPH antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10338-1-PBS in a different storage buffer formulation.

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  IP experiment of A375 using 10338-1-AP (same clone as 10338-1-PBS)

  IP result of anti-Melanophilin (IP:10338-1-AP, 4ug; Detection:10338-1-AP 1:600) with A375 cells lysate 3600ug. This data was developed using the same antibody clone with 10338-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 10338-1-AP (same clone as 10338-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 10338-1-AP (Melanophilin antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 10338-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 10338-1-AP (same clone as 10338-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 10338-1-AP (Melanophilin antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 10338-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 10338-1-AP (same clone as 10338-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Melanophilin antibody (10338-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10338-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

10338-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA MLPH und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MLPH fusion protein Ag0316
• Vollständiger Name: melanophilin
• Berechnetes Molekulargewicht: 66 kDa
• Beobachtetes Molekulargewicht: 66 kDa, 80 kDa
• GenBank-Zugangsnummer: BC001653
• Gene symbol: MLPH
• Gene ID (NCBI): 79083
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Melanophilin (Mlph) is a member of the exophilin subfamily of Rab effector proteins. MLPH is a modular protein, forming a ternary complex with the small Ras-related GTPase Rab27A in its GTP-bound form and the motor protein myosin Va. It regulates retention of melanosomes at the peripheral actin cytoskeleton of melanocytes, a process essential for normal mammalian pigmentation. Defects in MLPH are a cause of Griscelli syndrome type 3 (GS3), which is characterized by a silver-gray hair color and abnormal pigment distribution in the hair shaft.

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