MLPH Rekombinanter Antikörper

MLPH Rekombinant Antikörper für WB, IHC, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

241621G4

Kat-Nr. : 84282-1-PBS

Synonyme

241621G4, Exophilin 3, Exophilin-3, Melanophilin, SLAC2 A

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  WB analysis using 84282-1-RR (same clone as 84282-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84282-1-RR (MLPH antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84282-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP01196-3

  Sandwich ELISA standard curve of MP01196-3, Human MLPH Recombinant Matched Antibody Pair - PBS only. 84282-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag32931. 84282-4-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL.

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  Sample test of MP01196-3

  The mean MLPH concentration was determined to be 5.9 ng/mL in A549 cell extract based on a 1.5 mg/mL extract load and 1.7 ng/mL in HT-29 cell extract based on a 1.5 mg/mL extract load.

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  IHC staining of human skin cancer using 84282-1-RR (same clone as 84282-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 84282-1-RR (MLPH antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84282-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 84282-1-RR (same clone as 84282-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 84282-1-RR (MLPH antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84282-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84282-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84282-1-RR against Human MLPH were performed. The affinity constant is 2.43 nM.

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Geprüfte Anwendungen

Produktinformation

84282-1-PBS bindet in WB, IHC, Sandwich ELISA, Indirect ELISA, Sample test MLPH und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: MLPH fusion protein Ag32931
• Vollständiger Name: melanophilin
• Berechnetes Molekulargewicht: 66 kDa
• Beobachtetes Molekulargewicht: 66 kDa, 80 kDa
• GenBank-Zugangsnummer: BC001653
• Gene symbol: MLPH
• Gene ID (NCBI): 79083
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Melanophilin (Mlph) is a member of the exophilin subfamily of Rab effector proteins. MLPH is a modular protein, forming a ternary complex with the small Ras-related GTPase Rab27A in its GTP-bound form and the motor protein myosin Va. It regulates retention of melanosomes at the peripheral actin cytoskeleton of melanocytes, a process essential for normal mammalian pigmentation. Defects in MLPH are a cause of Griscelli syndrome type 3 (GS3), which is characterized by a silver-gray hair color and abnormal pigment distribution in the hair shaft.