MLPH Rekombinanter Antikörper

MLPH Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

241621G4

Kat-Nr. : 84282-1-RR

Synonyme

SLAC2A, SLAC2 A, Melanophilin, Exophilin-3, Exophilin 3

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  WB analysis using 84282-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84282-1-RR (MLPH antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human skin cancer using 84282-1-RR

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 84282-1-RR (MLPH antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin cancer using 84282-1-RR

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 84282-1-RR (MLPH antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 84282-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84282-1-RR against Human MLPH were performed. The affinity constant is 2.43 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HT-29-Zellen, LNCaP-Zellen, MCF-7-Zellen, PC-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Hautkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84282-1-RR bindet in WB, IHC, ELISA MLPH und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: MLPH fusion protein Ag32931
• Vollständiger Name: melanophilin
• Berechnetes Molekulargewicht: 66 kDa
• Beobachtetes Molekulargewicht: 66 kDa, 80 kDa
• GenBank-Zugangsnummer: BC001653
• Gene symbol: MLPH
• Gene ID (NCBI): 79083
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Melanophilin (Mlph) is a member of the exophilin subfamily of Rab effector proteins. MLPH is a modular protein, forming a ternary complex with the small Ras-related GTPase Rab27A in its GTP-bound form and the motor protein myosin Va. It regulates retention of melanosomes at the peripheral actin cytoskeleton of melanocytes, a process essential for normal mammalian pigmentation. Defects in MLPH are a cause of Griscelli syndrome type 3 (GS3), which is characterized by a silver-gray hair color and abnormal pigment distribution in the hair shaft.