MLXIPL Polyklonaler Antikörper

MLXIPL Polyklonal Antikörper für WB, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13256-1-PBS

Synonyme

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  WB analysis of HepG2 using 13256-1-AP (same clone as 13256-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13256-1-AP (MLXIPL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13256-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 13256-1-AP (same clone as 13256-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13256-1-AP (MLXIPL antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13256-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 13256-1-AP (same clone as 13256-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 13256-1-AP (MLXIPL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13256-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 13256-1-AP (same clone as 13256-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13256-1-AP (MLXIPL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13256-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human liver using 13256-1-AP (same clone as 13256-1-PBS)

  human liver tissue were subjected to SDS PAGE followed by western blot with 13256-1-AP (MLXIPL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13256-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HepG2 using 13256-1-AP (same clone as 13256-1-PBS)

  IP result of anti-MLXIPL (IP:13256-1-AP, 4ug; Detection:13256-1-AP 1:1000) with HepG2 cells lysate 3600ug. This data was developed using the same antibody clone with 13256-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

13256-1-PBS bindet in WB, IP, Indirect ELISA MLXIPL und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MLXIPL fusion protein Ag3481
• Vollständiger Name: MLX interacting protein-like
• Berechnetes Molekulargewicht: 852 aa, 93 kDa
• Beobachtetes Molekulargewicht: 65 kDa
• GenBank-Zugangsnummer: BC012925
• Gene symbol: MLXIPL
• Gene ID (NCBI): 51085
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Carbohydrate response element-binding protein (ChREBP), also known as MLXIPL, is a glucose-responsive transcription factor that has a fundamental role in the glucose-mediated induction of genes involved in hepatic glycolysis and lipogenesis. Circulating blood glucose levels affect ChREBP activity in hepatocytes largely by post-translational mechanisms that include phosphorylation-dependent subcellular localization [PMID:21665952]. It enhances expression of the liver pyruvate kinase (LPK) gene and all lipogenic enzyme genes resulting in the conversion of excess dietary carbohydrate into triglycerides that can be stored as fat [PMID:15118080]. Molecular Weight of ChREBP splice variants: 62/78/91/93 kDa.