MMAB Polyklonaler Antikörper

MMAB Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11137-1-PBS

Synonyme

---

• 

  WB analysis of HeLa using 11137-1-AP (same clone as 11137-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 11137-1-AP (MMAB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11137-1-PBS in a different storage buffer formulation.

• 

  WB analysis of BxPC-3 using 11137-1-AP (same clone as 11137-1-PBS)

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 11137-1-AP (MMAB antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11137-1-PBS in a different storage buffer formulation.

• 

  WB analysis of L02 using 11137-1-AP (same clone as 11137-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 11137-1-AP (MMAB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11137-1-PBS in a different storage buffer formulation.

• 

  WB analysis of L02 using 11137-1-AP (same clone as 11137-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 11137-1-AP (MMAB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11137-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 11137-1-AP (same clone as 11137-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11137-1-AP (MMAB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11137-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HeLa using 11137-1-AP (same clone as 11137-1-PBS)

  IP result of anti-MMAB (IP:11137-1-AP, 4ug; Detection:11137-1-AP 1:500) with HeLa cells lysate 1200ug. This data was developed using the same antibody clone with 11137-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 11137-1-AP (same clone as 11137-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11137-1-AP (MMAB antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11137-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 11137-1-AP (same clone as 11137-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MMAB antibody (11137-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 11137-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

11137-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA MMAB und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MMAB fusion protein Ag1602
• Vollständiger Name: methylmalonic aciduria (cobalamin deficiency) cblB type
• Berechnetes Molekulargewicht: 27 kDa
• Beobachtetes Molekulargewicht: 27 kDa
• GenBank-Zugangsnummer: BC011831
• Gene symbol: MMAB
• Gene ID (NCBI): 326625
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The MMAB gene encodes cob(I)alamin adenosyltransferase, which catalyzes the final step in the synthesis of the coenzyme adenosylcobalamin (AdoCbl). AdoCbl is a vitamin B12-containing coenzyme for methylmalonyl-CoA mutase. The deduced 250-amino acid protein has a calculated molecular mass of 27.3 kD.