MME,CD10 Polyklonaler Antikörper

MME,CD10 Polyklonal Antikörper für FC, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Rezensionen (3)

Kat-Nr. : 10302-1-AP

Synonyme

Atriopeptidase, CALLA, CD10, Enkephalinase, EPN, membrane metallo endopeptidase, MME, MME,CD10, NEP, Neprilysin, Neutral endopeptidase, Neutral endopeptidase 24.11

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Galerie der Validierungsdaten

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  WB analysis using 10302-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10302-1-AP (MME,CD10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 10302-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10302-1-AP (MME,CD10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10302-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10302-1-AP (MME,CD10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 10302-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10302-1-AP (MME,CD10 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 10302-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10302-1-AP (MME,CD10 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human renal cell carcinoma using 10302-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma using 10302-1-AP (MME,CD10 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human renal cell carcinoma using 10302-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma using 10302-1-AP (MME,CD10 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human liver cancer using 10302-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 10302-1-AP (MME,CD10 antibody) at dilution of 1:50 (under 10x lens). Heat mediated antigen retrieved with Citric acid buffer, pH6.0.

• 

  IHC staining of human liver cancer using 10302-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 10302-1-AP (MME,CD10 antibody) at dilution of 1:50 (under 40x lens). Heat mediated antigen retrieved with Citric acid buffer, pH6.0.

• 

  IHC staining of human kidney using 10302-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 10302-1-AP (MME,CD10 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 10302-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 10302-1-AP (MME,CD10 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 10302-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 10302-1-AP (MME,CD10 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 10302-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 10302-1-AP (MME,CD10 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human hepatocirrhosis using 10302-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 10302-1-AP (MME,CD10 antibody) at dilution of 1:50 (under 10x lens). Heat mediated antigen retrieved with Citric acid buffer, pH6.0.

• 

  IHC staining of human hepatocirrhosis using 10302-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 10302-1-AP (MME,CD10 antibody) at dilution of 1:50 (under 40x lens). Heat mediated antigen retrieved with Citric acid buffer, pH6.0.

• 

  IHC staining of human hepatocirrhosis using 10302-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 10302-1-AP (MME,CD10 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human hepatocirrhosis using 10302-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 10302-1-AP (MME,CD10 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human renal cell carcinoma using 10302-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma using 10302-1-AP (MME,CD10 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human renal cell carcinoma using 10302-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma using 10302-1-AP (MME,CD10 antibody) at dilution of 1:50 (under 40x lens).

• 

  FC experiment of Jurkat using 10302-1-AP

  1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human MME,CD10 (10302-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausnierengewebe, HEK-293-Zellen, Rattennierengewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Leberkarzinomgewebe, humanes Leberzirrhosegewebe, humanes Nierenzellkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

10302-1-AP bindet in WB, IHC, FC, ELISA MME,CD10 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, hamster, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MME,CD10 fusion protein Ag0427
• Vollständiger Name: membrane metallo-endopeptidase
• Berechnetes Molekulargewicht: 750 aa, 86 kDa
• Beobachtetes Molekulargewicht: 90-100 kDa
• GenBank-Zugangsnummer: BC101658
• Gene symbol: MME
• Gene ID (NCBI): 4311
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MME is also names as neprilysin,CALLA,NEP,SFE,CD10 and belongs to the peptidase M13 family.It is a glycoprotein that is particularly abundant in kidney, where it is present on the brush border of proximal tubules and on glomerular epithelium.CD10 has been considered a useful marker in the diagnosis of renal carcinomas, because of its expression in clear cell and papillary renal cell carcinomas and its absence in chromophobe renal cell carcinomas(PMID:15286660).

Publikationen

Species	Application	Title
mouse	WB	J Pathol; Association of dysfunctional synapse defective 1 (SYDE1) with restricted fetal growth - SYDE1 regulates placental cell migration and invasion.; Authors - Hsiao-Fan Lo
human,hamster	WB	Fitoterapia; A novel peptidoglycan isolated from Semiaquilegia adoxoides inhibits Aβ42 production via activating autophagy; Authors - Saijuan Li

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Luca (Verified Customer) (09-12-2022)

The antibody unfortunately did not work but most likely the conditions of the WB need optimized.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000

FH

Luca (Verified Customer) (06-10-2022)

I unfortunately could not detect a band against this protein even when expressed using a plasmid, but I have to better optimize my conditions. I liked having the facts sheet with me as that was helpful, so thank you for including it with the Ab.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500- 1:1000

FH

Boyan (Verified Customer) (10-27-2021)

This antibody recognised a clean band around 100 kd.

• Applications: Western Blot