Neprilysin/CD10 Polyklonaler Antikörper

Neprilysin/CD10 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Rezensionen (3)

Kat-Nr. : 10302-1-AP

Synonyme

MME, Neprilysin, Atriopeptidase, CALLA, CD10

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  WB analysis of mouse kidney using 10302-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10302-1-AP (Neprilysin/CD10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 10302-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10302-1-AP (Neprilysin/CD10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10302-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10302-1-AP (Neprilysin/CD10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 10302-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10302-1-AP (Neprilysin/CD10 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 10302-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10302-1-AP (Neprilysin/CD10 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human kidney using 10302-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 10302-1-AP (Neprilysin/CD10 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 10302-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 10302-1-AP (Neprilysin/CD10 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human renal cell carcinoma using 10302-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma using 10302-1-AP (Neprilysin/CD10 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human renal cell carcinoma using 10302-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma using 10302-1-AP (Neprilysin/CD10 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human liver cancer using 10302-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 10302-1-AP (Neprilysin/CD10 antibody) at dilution of 1:50 (under 10x lens). Heat mediated antigen retrieved with Citric acid buffer, pH6.0.

• 

  IHC staining of human liver cancer using 10302-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 10302-1-AP (Neprilysin/CD10 antibody) at dilution of 1:50 (under 40x lens). Heat mediated antigen retrieved with Citric acid buffer, pH6.0.

• 

  IHC staining of human hepatocirrhosis using 10302-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 10302-1-AP (Neprilysin/CD10 antibody) at dilution of 1:50 (under 10x lens). Heat mediated antigen retrieved with Citric acid buffer, pH6.0.

• 

  IHC staining of human hepatocirrhosis using 10302-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 10302-1-AP (Neprilysin/CD10 antibody) at dilution of 1:50 (under 40x lens). Heat mediated antigen retrieved with Citric acid buffer, pH6.0.

• 

  IHC staining of human renal cell carcinoma using 10302-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma using 10302-1-AP (Neprilysin/CD10 antibody) at dilutionNeprilysin/CD10:50 (under 10x lens).

• 

  IHC staining of human renal cell carcinoma using 10302-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma using 10302-1-AP (Neprilysin/CD10 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of MCF-7 using 10302-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using Neprilysin/CD10 antibody (10302-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausnierengewebe, HEK-293-Zellen, Rattennierengewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Leberzirrhosegewebe, humanes Leberkarzinomgewebe, humanes Nierenzellkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

10302-1-AP bindet in WB, IHC, IF/ICC, ELISA Neprilysin/CD10 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, hamster, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Neprilysin/CD10 fusion protein Ag0427
• Vollständiger Name: membrane metallo-endopeptidase
• Berechnetes Molekulargewicht: 750 aa, 86 kDa
• Beobachtetes Molekulargewicht: 90-100 kDa
• GenBank-Zugangsnummer: BC101658
• Gene symbol: CD10
• Gene ID (NCBI): 4311
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD10, also known as neprilysin, membrane metallo-endopeptidase (MME), neutral endopeptidase (NEP), or common acute lymphoblastic leukemia antigen (CALLA), is a 100-kDa type II transmembrane glycoprotein belonging to peptidase M13 family (PMID: 7760013; 8102558). Among hematopoietic cells, CD10 is expressed on granulocytes, B cell precursors, mature germinal center B cells, a subset of immature thymocytes (PMID: 13679451). CD10 is also expressed on a variety of nonhematopoietic cell types, including bronchial epithelial cells, cultured fibroblasts, bone marrow stromal cells, renal proximal tubular epithelial cells, breast myoepithelium, biliary canaliculi (PMID: 8102558). CD10 is a cell surface peptidase that cleaves peptide bonds on the amino side of hydrophobic amino acids and inactivates a variety of physiologically active peptides. Loss or decreases in CD10 expression have been reported in a variety of malignancies (PMID: 16054017).

Publikationen

Species	Application	Title
mouse	WB	J Pathol; Association of dysfunctional synapse defective 1 (SYDE1) with restricted fetal growth - SYDE1 regulates placental cell migration and invasion.; Authors - Hsiao-Fan Lo
human,hamster	WB	Fitoterapia; A novel peptidoglycan isolated from Semiaquilegia adoxoides inhibits Aβ42 production via activating autophagy; Authors - Saijuan Li

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Luca (Verified Customer) (09-12-2022)

The antibody unfortunately did not work but most likely the conditions of the WB need optimized.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000

FH

Luca (Verified Customer) (06-10-2022)

I unfortunately could not detect a band against this protein even when expressed using a plasmid, but I have to better optimize my conditions. I liked having the facts sheet with me as that was helpful, so thank you for including it with the Ab.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500- 1:1000

FH

Boyan (Verified Customer) (10-27-2021)

This antibody recognised a clean band around 100 kd.

• Applications: Western Blot

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