Neprilysin/CD10 Rekombinanter Antikörper

Neprilysin/CD10 Rekombinant Antikörper für WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241322A8

Kat-Nr. : 84181-1-PBS

Synonyme

MME, Neprilysin, 241322A8, Atriopeptidase, CALLA

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  WB analysis of Raji using 84181-1-RR (same clone as 84181-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 84181-1-RR (Neprilysin/CD10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84181-1-PBS in a different storage buffer formulation.

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  WB analysis using 84181-1-RR (same clone as 84181-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84181-1-RR (Neprilysin/CD10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84181-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 84181-1-RR (same clone as 84181-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84181-1-RR (Neprilysin/CD10 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84181-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 84181-1-RR (same clone as 84181-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84181-1-RR (Neprilysin/CD10 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84181-1-PBS in a different storage buffer formulation.

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  IHC staining of Human Kidney(renal cell) Cancer using 84181-1-RR (same clone as 84181-1-PBS)

  Immunohistochemical analysis of paraffin-embedded Human Kidney(renal cell) Cancer slide using 84181-1-RR (Neprilysin/CD10 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84181-1-PBS in a different storage buffer formulation.

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  IHC staining of Human Kidney(renal cell) Cancer using 84181-1-RR (same clone as 84181-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney(renal cell) cancer slide using 84181-1-RR (Neprilysin/CD10 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84181-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse kidney using 84181-1-RR (same clone as 84181-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using Neprilysin/CD10 antibody (84181-1-RR, Clone: 241322A8 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84181-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01076-1

  Cytometric bead array standard curve of MP01076-1, MME,CD10 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84181-3-PBS. Detection antibody: 84181-1-PBS. Standard: RP02332. Range: 0.781-100 ng/mL.

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  Cytometric bead array standard curve of MP01076-3

  Cytometric bead array standard curve of MP01076-3, MME,CD10 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84181-2-PBS. Detection antibody: 84181-1-PBS. Standard: RP02332. Range: 0.781-100 ng/mL.

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  Affinity and Kinetic Characterization of 84181-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84181-1-RR against Human Neprilysin/CD10 were performed. The affinity constant is 3.07 nM.

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Geprüfte Anwendungen

Produktinformation

84181-1-PBS bindet in WB, IHC, IF-P, Cytometric bead array, Indirect ELISA Neprilysin/CD10 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: membrane metallo-endopeptidase
• Berechnetes Molekulargewicht: 750 aa, 86 kDa
• Beobachtetes Molekulargewicht: 100 kDa
• GenBank-Zugangsnummer: BC101658
• Gene symbol: CD10
• Gene ID (NCBI): 4311
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD10, also known as neprilysin, membrane metallo-endopeptidase (MME), neutral endopeptidase (NEP), or common acute lymphoblastic leukemia antigen (CALLA), is a 100-kDa type II transmembrane glycoprotein belonging to peptidase M13 family (PMID: 7760013; 8102558). Among hematopoietic cells, CD10 is expressed on granulocytes, B cell precursors, mature germinal center B cells, a subset of immature thymocytes (PMID: 13679451). CD10 is also expressed on a variety of nonhematopoietic cell types, including bronchial epithelial cells, cultured fibroblasts, bone marrow stromal cells, renal proximal tubular epithelial cells, breast myoepithelium, biliary canaliculi (PMID: 8102558). CD10 is a cell surface peptidase that cleaves peptide bonds on the amino side of hydrophobic amino acids and inactivates a variety of physiologically active peptides. Loss or decreases in CD10 expression have been reported in a variety of malignancies (PMID: 16054017).