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MME,CD10 Rekombinanter Antikörper

MME,CD10 Rekombinant Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241322D7

Kat-Nr. : 84181-4-PBS

Synonyme

MME, Neprilysin, Neprilysin/CD10, Enkephalinase, EC:3.4.24.11

Filter:
  • Western Blot (WB) analysis of various lysates using MME,CD10 Recombinant antibody (84181-4-RR)

    WB analysis using 84181-4-RR (same clone as 84181-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84181-4-RR (MME,CD10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84181-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84181-4-RR

    Affinity and Kinetic Characterization of 84181-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84181-4-RR against Human MME,CD10 were performed. The affinity constant is 11.4 nM.

Geprüfte Anwendungen

Produktinformation

84181-4-PBS bindet in WB, Indirect ELISA MME,CD10 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen Rekombinantes Protein
Vollständiger Name membrane metallo-endopeptidase
Berechnetes Molekulargewicht 750 aa, 86 kDa
Beobachtetes Molekulargewicht 100 kDa
GenBank-ZugangsnummerBC101658
Gene symbol CD10
Gene ID (NCBI) 4311
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein A purfication
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD10, also known as neprilysin, membrane metallo-endopeptidase (MME), neutral endopeptidase (NEP), or common acute lymphoblastic leukemia antigen (CALLA), is a 100-kDa type II transmembrane glycoprotein belonging to peptidase M13 family (PMID: 7760013; 8102558). Among hematopoietic cells, CD10 is expressed on granulocytes, B cell precursors, mature germinal center B cells, a subset of immature thymocytes (PMID: 13679451). CD10 is also expressed on a variety of nonhematopoietic cell types, including bronchial epithelial cells, cultured fibroblasts, bone marrow stromal cells, renal proximal tubular epithelial cells, breast myoepithelium, biliary canaliculi (PMID: 8102558). CD10 is a cell surface peptidase that cleaves peptide bonds on the amino side of hydrophobic amino acids and inactivates a variety of physiologically active peptides. Loss or decreases in CD10 expression have been reported in a variety of malignancies (PMID: 16054017).