MMP-9 (N-terminal) Polyklonaler Antikörper

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• KD/KO Validated

MMP-9 (N-terminal) Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10375-2-PBS

Synonyme

MMP9 (N-terminal), MMP9, 67 kDa matrix metalloproteinase-9, 82 kDa matrix metalloproteinase-9, CLG4B

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  WB analysis using 10375-2-AP (same clone as 10375-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10375-2-AP (MMP9 (N-terminal) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10375-2-PBS in a different storage buffer formulation.

• 

  WB analysis using 10375-2-AP (same clone as 10375-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10375-2-AP (MMP9 (N-terminal) antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10375-2-PBS in a different storage buffer formulation.

• 

  WB analysis using 10375-2-AP (same clone as 10375-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10375-2-AP (MMP-9 (N-terminal) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10375-2-PBS in a different storage buffer formulation.

• 

  WB analysis using 10375-2-AP (same clone as 10375-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10375-2-AP (MMP9 (N-terminal) antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10375-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 10375-2-AP (same clone as 10375-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10375-2-AP (MMP9 (N-terminal) antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10375-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 10375-2-AP (same clone as 10375-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10375-2-AP (MMP9 (N-terminal) antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10375-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 10375-2-AP (same clone as 10375-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10375-2-AP (MMP9 (N-terminal) antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 10375-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 10375-2-AP (same clone as 10375-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10375-2-AP (MMP9 (N-terminal) antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 10375-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 10375-2-AP (same clone as 10375-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10375-2-AP (MMP9 (N-terminal) antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10375-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 10375-2-AP (same clone as 10375-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10375-2-AP (MMP9 (N-terminal) antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10375-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 10375-2-AP (same clone as 10375-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10375-2-AP (MMP9 (N-terminal) antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10375-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 10375-2-AP (same clone as 10375-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10375-2-AP (MMP9 (N-terminal) antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10375-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 10375-2-AP (same clone as 10375-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10375-2-AP (MMP9 (N-terminal) antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10375-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 10375-2-AP (same clone as 10375-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10375-2-AP (MMP9 (N-terminal) antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10375-2-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 10375-2-AP (same clone as 10375-2-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MMP9 (N-terminal) antibody (10375-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 10375-2-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 10375-2-AP (same clone as 10375-2-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human MMP9 (N-terminal) (10375-2-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 10375-2-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

10375-2-PBS bindet in WB, IHC, IF/ICC, FC (Intra), ELISA MMP-9 (N-terminal) und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MMP-9 (N-terminal) fusion protein Ag0552
• Vollständiger Name: matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)
• Berechnetes Molekulargewicht: 707 aa, 78 kDa
• Beobachtetes Molekulargewicht: 92 kDa, 67 kDa
• GenBank-Zugangsnummer: BC006093
• Gene symbol: MMP-9
• Gene ID (NCBI): 4318
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, tissue remodeling, and disease processes, such as arthritis or metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. Matrix metalloproteinase 9 (gelatinase B, 92 kDa gelatinase, 92 kDa type IV collagenase) (MMP9, synonyms: GELB, CLG4B) degrades collagens type IV and V. Studies in rhesus monkeys suggest that MMP9 is involved in IL-8-induced mobilization hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. The pro-MMP9 is 92 kDa, and it can be detected a processed form of 68 kDa. This protein can exist as a dimer of 180 kDa (PMID:7492685).

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