MMP1 Rekombinanter Antikörper

MMP1 Rekombinant Antikörper für Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

230343B6

Kat-Nr. : 83114-3-PBS

Synonyme

22 kDa interstitial collagenase, 27 kDa interstitial collagenase, CLG, CLGN, EC:3.4.24.7

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  Sandwich ELISA standard curve of MP00290-1

  Sandwich ELISA standard curve of MP00290-1, MMP1 Recombinant Matched Antibody Pair, PBS Only. 83114-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard  Eg0527. 83114-3-PBS was HRP conjugated as the detection antibody. Range: 19.5-1250 pg/mL.

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  Sample test of MP00290-1

  Plasma of fourteen individual healthy human donors were measured. The human MMP1 concentration of detected samples was determined to be 653.4 pg/mL with a range of 99.3 - 1,631.8 pg/mL.

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  Sample test of MP00290-1

  HT1080 were cultured in DMEM supplemented with 10% fetal bovine serum, 2.5 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernate was removed, assayed for human MMP1, and measured 907.5 pg/mL.

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  Sample test of MP00290-1

  Serum of fourteen individual healthy human donors were measured. The human MMP1 concentration of detected samples was determined to be 9,124.3 pg/mL with a range of 3,045.5 - 19,864.3 pg/mL.

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  Cytometric bead array standard curve of MP00290-1

  Cytometric bead array standard curve of MP00290-1, MMP1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83114-4-PBS. Detection antibody: 83114-3-PBS. Standard: Eg0527. Range: 0.625-20 ng/mL.

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  Affinity and Kinetic Characterization of 83114-3-PBS

  Biolayer interferometry (BLl) kinetic assay of 83114-3-PBS against Human MMP1 was performed. The affinity constant is 12.7 nM.

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Geprüfte Anwendungen

Produktinformation

83114-3-PBS bindet in Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test MMP1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: MMP1 fusion protein Eg0527
• Vollständiger Name: matrix metallopeptidase 1 (interstitial collagenase)
• Berechnetes Molekulargewicht: 54 kDa
• GenBank-Zugangsnummer: BC013875
• Gene symbol: MMP1
• Gene ID (NCBI): 4312
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.