MMP11 Polyklonaler Antikörper

MMP11 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 30615-1-AP

Synonyme

Matrix metalloproteinase 11, MMP 11, STMY3, Stromelysin 3

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  WB analysis using 30615-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30615-1-AP (MMP11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of A549 using 30615-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using MMP11 antibody (30615-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of MCF-7 using 30615-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using MMP11 antibody (30615-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, A549-Zellen, T-47D-Zellen
• Erfolgreiche Detektion in IF/ICC: A549-Zellen, MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

30615-1-AP bindet in WB, IF/ICC, ELISA MMP11 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MMP11 fusion protein Ag31191
• Vollständiger Name: matrix metallopeptidase 11 (stromelysin 3)
• Berechnetes Molekulargewicht: 55 kDa
• Beobachtetes Molekulargewicht: 50-55 kDa
• GenBank-Zugangsnummer: NM_005940
• Gene symbol: MMP11
• Gene ID (NCBI): 4320
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Matrix metalloproteinase 11 (MMP11), also named stromelysin-3, is a member of the stromelysin subgroup belonging to MMPs superfamily. The role of MMP11 in cancer progression has been shown by several preclinical observations: its expression promotes tumor take in mice, homing of malignant epithelial cells, cancer progression by remodeling extracellular matrix, and antiapoptotic and antinecrotic effect on tumor cells (PMID: 19509157, 26892540). MMP11 has a calculated molecular mass of 55 kDa.

Publikationen

Species	Application	Title
human	IF	Cancer Cell; Single-cell multi-stage spatial evolutional map of esophageal carcinogenesis; Authors - Jiang Chang