MMP12 Monoklonaler Antikörper

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• KD/KO Validated

MMP12 Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

CloneNo.

1C6B5

Kat-Nr. : 66930-1-Ig

Synonyme

HME, Macrophage elastase, Macrophage metalloelastase, Matrix metalloproteinase 12, ME, MME, MMP 12, MMP12

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  WB analysis of A549 using 66930-1-Ig

  WB result of MMP12 antibody (66930-1-Ig; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-MMP12 transfected A549 cells.

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  WB analysis using 66930-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66930-1-Ig (MMP12 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A549-Zellen, HEK-293-Zellen, NCI-H1299-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 3 publications below

Produktinformation

66930-1-Ig bindet in WB, IHC, ELISA MMP12 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: MMP12 fusion protein Ag19068
• Vollständiger Name: matrix metallopeptidase 12 (macrophage elastase)
• Berechnetes Molekulargewicht: 470 aa, 54 kDa
• Beobachtetes Molekulargewicht: 54 kDa, 45 kDa
• GenBank-Zugangsnummer: BC112301
• Gene symbol: MMP12
• Gene ID (NCBI): 4321
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MMP12(Matrix metalloproteinase-12) is a 54 kDa proenzyme that is processed into a 45 kDa and then a 22 kDa active form(15723202). MMP9 and MMP12 promote intimal thickening by independent cleavage of N-cadherin, which elevates vascular smooth muscle cell proliferation via beta-catenin signalling. Its overexpression in myeloid lineage cells plays a key role in modulating myelopoiesis, immune suppression, and lung tumorigenesis(PMID: 21378275).

Publikationen

Species	Application	Title
mouse	IHC	Eur J Pharmacol; Reversal of elastase-induced abdominal aortic aneurysm following the delivery of nanoparticle-based pentagalloyl glucose (PGG) is associated with reduced inflammatory and immune markers.; Authors - Saphala Dhital
mouse	WB	Drug Des Devel Ther; Identification of Active Compounds and Mechanism of Huangtu Decoction for the Treatment of Ulcerative Colitis by Network Pharmacology Combined with Experimental Verification; Authors - Wenwen Chen
human,mouse	IHC	Cytokine; IL-33/ST2 enhances MMP-12 expression by macrophages to mediate inflammatory and immune response in IgG4-Related Ophthalmic Disease; Authors - Xia Ding
human	WB,IHC	Curr Eye Res; Cohesin Complex Interacting with Promoters of MMP Genes for in Pterygium Occurrence; Authors - Shichao Han