MMP12 Monoklonaler Antikörper

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• KD/KO Validated

MMP12 Monoklonal Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1C6B5

Kat-Nr. : 66930-1-PBS

Synonyme

HME, Macrophage elastase, Macrophage metalloelastase, Matrix metalloproteinase 12, ME, MME, MMP 12, MMP12

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  WB analysis using 66930-1-Ig (same clone as 66930-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66930-1-Ig (MMP12 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66930-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 66930-1-Ig (same clone as 66930-1-PBS)

  WB result of MMP12 antibody (66930-1-Ig; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-MMP12 transfected A549 cells. This data was developed using the same antibody clone with 66930-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66930-1-PBS bindet in WB, Indirect ELISA MMP12 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: MMP12 fusion protein Ag19068
• Vollständiger Name: matrix metallopeptidase 12 (macrophage elastase)
• Berechnetes Molekulargewicht: 470 aa, 54 kDa
• Beobachtetes Molekulargewicht: 54 kDa, 45 kDa
• GenBank-Zugangsnummer: BC112301
• Gene symbol: MMP12
• Gene ID (NCBI): 4321
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MMP12(Matrix metalloproteinase-12) is a 54 kDa proenzyme that is processed into a 45 kDa and then a 22 kDa active form(15723202). MMP9 and MMP12 promote intimal thickening by independent cleavage of N-cadherin, which elevates vascular smooth muscle cell proliferation via beta-catenin signalling. Its overexpression in myeloid lineage cells plays a key role in modulating myelopoiesis, immune suppression, and lung tumorigenesis(PMID: 21378275).