MMP13 Polyklonaler Antikörper

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• KD/KO Validated

MMP13 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 18165-1-PBS

Synonyme

CLG3, Collagenase 3, EC:3.4.24.-, Matrix metalloproteinase 13, Matrix metalloproteinase-13

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  WB analysis using 18165-1-AP (same clone as 18165-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 18165-1-AP (MMP13 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18165-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 18165-1-AP (same clone as 18165-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 18165-1-AP (MMP13 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18165-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 18165-1-AP (same clone as 18165-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 18165-1-AP (MMP13 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18165-1-PBS in a different storage buffer formulation.

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  IP experiment of MCF-7 using 18165-1-AP (same clone as 18165-1-PBS)

  IP result of anti-MMP13 (IP:18165-1-AP, 4ug; Detection:18165-1-AP 1:300) with MCF-7 cells lysate 2480 ug. This data was developed using the same antibody clone with 18165-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 18165-1-AP (same clone as 18165-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18165-1-AP (MMP13 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18165-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 18165-1-AP (same clone as 18165-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18165-1-AP (MMP13 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18165-1-PBS in a different storage buffer formulation.

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  IHC staining of human hepatocirrhosis using 18165-1-AP (same clone as 18165-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 18165-1-AP (MMP13 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18165-1-PBS in a different storage buffer formulation.

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  IHC staining of human hepatocirrhosis using 18165-1-AP (same clone as 18165-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 18165-1-AP (MMP13 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18165-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 18165-1-AP (same clone as 18165-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 18165-1-AP (MMP13 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18165-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 18165-1-AP (same clone as 18165-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 18165-1-AP (MMP13 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18165-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 18165-1-AP (same clone as 18165-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18165-1-AP (MMP13 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18165-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 18165-1-AP (same clone as 18165-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18165-1-AP (MMP13 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18165-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 18165-1-AP (same clone as 18165-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 18165-1-AP (MMP13 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 18165-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 18165-1-AP (same clone as 18165-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 18165-1-AP (MMP13 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 18165-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 18165-1-AP (same clone as 18165-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using MMP13 antibody (18165-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18165-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

18165-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA MMP13 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MMP13 fusion protein Ag12653
• Vollständiger Name: matrix metallopeptidase 13
• Berechnetes Molekulargewicht: 471 aa, 54 kDa
• Beobachtetes Molekulargewicht: 65-70 kDa, 50-55 kDa, 40 kDa
• GenBank-Zugangsnummer: BC074808
• Gene symbol: MMP13
• Gene ID (NCBI): 4322
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MMP-13(Matrix metalloproteinase-13), cleaves type I collagen and was previously thought to be the rodent analogue of human interstitial MMP-1. MMP-13 is found in hypertrophic and calcifying cartilage of mammalian growth plate. The bands seen with rat MMP-13 are consistent with a 59-kDa latent form and a 43-kDa active form reported for this enzyme, along with a smaller band at approximately 30 kDa(PMID:10771523). It also can be detected 3 bands that the glycosylated proMMP-13 (70 kDa), unglycosylated proform (55 kDa), and active MMP-13 enzyme (40kDa) by western blot(PMID:18332263).