MMP13 Rekombinanter Antikörper

MMP13 Rekombinant Antikörper für IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

240060B10

Kat-Nr. : 83188-2-PBS

Synonyme

240060B10, CLG3, Collagenase 3, EC:3.4.24.-, Matrix metalloproteinase 13

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  Sandwich ELISA standard curve of MP00266-1

  Sandwich ELISA standard curve of MP00266-1, MMP13 Recombinant Matched Antibody Pair, PBS Only. 83188-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard  Ag34892. 83188-2-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL.

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  IF Staining of MCF-7 using 83188-2-RR (same clone as 83188-2-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using MMP13 antibody (83188-2-RR, Clone: 240060B10 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83188-2-PBS in a different storage buffer formulation.

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  FC experiment of MCF-7 using 83188-2-RR (same clone as 83188-2-PBS)

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug MMP13 Recombinant antibody (83188-2-RR, Clone:240060B10) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83188-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00266-1

  Cytometric bead array standard curve of MP00266-1, MMP13 Recombinant Matched Antibody Pair, PBS Only. PBS Only. Capture antibody: 83188-3-PBS. Detection antibody: 83188-2-PBS. Standard: Ag34892. Range: 5-40 ng/mL.

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  Affinity and Kinetic Characterization of 83188-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83188-2-RR against Human MMP13 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

83188-2-PBS bindet in IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA MMP13 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: MMP13 fusion protein Ag34892
• Vollständiger Name: matrix metallopeptidase 13
• Berechnetes Molekulargewicht: 471 aa, 54 kDa
• Beobachtetes Molekulargewicht: 70 kDa, 54 kDa
• GenBank-Zugangsnummer: BC074808
• Gene symbol: MMP13
• Gene ID (NCBI): 4322
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MMP-13(Matrix metalloproteinase-13), cleaves type I collagen and was previously thought to be the rodent analogue of human interstitial MMP-1. MMP-13 is found in hypertrophic and calcifying cartilage of mammalian growth plate. The bands seen with rat MMP-13 are consistent with a 59-kDa latent form and a 43-kDa active form reported for this enzyme, along with a smaller band at approximately 30 kDa(PMID:10771523).