MMP14 / MT1-MMP Polyklonaler Antikörper

MMP14 / MT1-MMP Polyklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

27

Kat-Nr. : 14552-1-AP

Synonyme

Matrix metalloproteinase 14, MMP 14, MMP X1, MMP14, MT MMP 1, MT1 MMP, MT1MMP, MT1-MMP, MTMMP1

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Galerie der Validierungsdaten

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  WB analysis of HepG2 using 14552-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14552-1-AP (MT1-MMP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 14552-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 14552-1-AP (MT1-MMP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 14552-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 14552-1-AP (MT1-MMP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human colon using 14552-1-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 14552-1-AP (MT1-MMP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 14552-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 14552-1-AP (MT1-MMP antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of COLO 320 using 14552-1-AP

  Immunofluorescent analysis of COLO 320 cells using 14552-1-AP (MT1-MMP antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, A549-Zellen, COLO 320-Zellen, humanes Kolongewebe
• Erfolgreiche Detektion in IF: HepG2-Zellen, COLO 320-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 21 publications below
• IHC: See 5 publications below
• IF: See 3 publications below

Produktinformation

14552-1-AP bindet in WB, IHC, IF, ELISA MMP14 / MT1-MMP und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MMP14 / MT1-MMP fusion protein Ag5965
• Vollständiger Name: matrix metallopeptidase 14 (membrane-inserted)
• Berechnetes Molekulargewicht: 66 kDa
• Beobachtetes Molekulargewicht: 66 kDa, 45-50 kDa
• GenBank-Zugangsnummer: BC064803
• Gene symbol: MMP14
• Gene ID (NCBI): 4323
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MMP14, also named as MT1-MMP, is a key matrix metalloproteinase (MMP) family member which plays a crucial role in tumor growth, invasion and metastasis. MT1-MMP is a cell membrane-bound proteinase, and it enhances degradation of collagen IV, a major component of the basement membrane, by forming a complex with tissue inhibitor of metalloproteinase-2 (TIMP-2) to activate pro-MMP-2. MT1-MMP can influence venous invasion, intrahepatic metastasis , and patient outcome in hepatocellular carcinoma (HCC). MT1-MMP was reported to be present in centromere and could lead to chromosome instability in MDCK cells, indicating that MT1-MMP may have more novel functions in the intracellular compartments. In western blotting, pro-MMP14 (65 kDa) and MMP14 (51 kDa) bands showed with the truncated MMP14 (45, 42, 35, 20 kDa) forms (PMID:12097451).

Publikationen

Species	Application	Title
mouse	WB	Mol Ther; METTL3-mediated m6A modification of TIMP2 mRNA promotes podocyte injury in diabetic nephropathy.; Authors - Ling Jiang
human	WB	Int J Mol Sci; PLOD2 Is a Prognostic Marker in Glioblastoma That Modulates the Immune Microenvironment and Tumor Progression.; Authors - Nina Kreße
human	WB	Int J Mol Sci; LncRNA H19-Derived miR-675-5p Accelerates the Invasion of Extravillous Trophoblast Cells by Inhibiting GATA2 and Subsequently Activating Matrix Metalloproteinases.; Authors - Manabu Ogoyama
human	WB	Cancers (Basel); WISP2/CCN5 Suppresses Vasculogenic Mimicry through Inhibition of YAP/TAZ Signaling in Breast Cancer Cells.; Authors - Nathalie Ferrand
human	WB	Cancer Lett; Long non-coding RNA MALAT1 promotes gastric cancer tumorigenicity and metastasis by regulating vasculogenic mimicry and angiogenesis.; Authors - Yue Li
human	IHC	Aging (Albany NY); GABARAP suppresses EMT and breast cancer progression via the AKT/mTOR signaling pathway.; Authors - Ying Liu