MMP14 / MT1-MMP Polyklonaler Antikörper

MMP14 / MT1-MMP Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(36)

Kat-Nr. : 14552-1-AP

Synonyme

MMP14, Membrane-type-1 matrix metalloproteinase, Membrane-type matrix metalloproteinase 1, Matrix metalloproteinase-14, Matrix metalloproteinase 14



Geprüfte Anwendungen

Erfolgreiche Detektion in WBA549-Zellen, humanes Kolongewebe
Erfolgreiche Detektion in IF/ICCHepG2-Zellen, COLO 320-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:1000
Immunfluoreszenz (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

14552-1-AP bindet in WB, IHC, IF/ICC, ELISA MMP14 / MT1-MMP und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen MMP14 / MT1-MMP fusion protein Ag5965
Vollständiger Name matrix metallopeptidase 14 (membrane-inserted)
Berechnetes Molekulargewicht 66 kDa
Beobachtetes Molekulargewicht 66 kDa, 45-50 kDa
GenBank-ZugangsnummerBC064803
Gene symbol MMP14
Gene ID (NCBI) 4323
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MMP14, also named as MT1-MMP, is a key matrix metalloproteinase (MMP) family member which plays a crucial role in tumor growth, invasion and metastasis. MT1-MMP is a cell membrane-bound proteinase, and it enhances degradation of collagen IV, a major component of the basement membrane, by forming a complex with tissue inhibitor of metalloproteinase-2 (TIMP-2) to activate pro-MMP-2. MT1-MMP can influence venous invasion, intrahepatic metastasis , and patient outcome in hepatocellular carcinoma (HCC). MT1-MMP was reported to be present in centromere and could lead to chromosome instability in MDCK cells, indicating that MT1-MMP may have more novel functions in the intracellular compartments. In western blotting, pro-MMP14 (65 kDa) and MMP14 (51 kDa) bands showed with the truncated MMP14 (45, 42, 35, 20 kDa) forms (PMID:12097451).

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for MMP14 / MT1-MMP antibody 14552-1-APProtokoll herunterladen
IF protocol for MMP14 / MT1-MMP antibody 14552-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
human,ratWB,IF

J Neuroinflammation

SPOCK2 modulates neuropathic pain by interacting with MT1-MMP to regulate astrocytic MMP-2 activation in rats with chronic constriction injury

Authors - Chenglong Wang
mouseWB

Mol Ther

METTL3-mediated m6A modification of TIMP2 mRNA promotes podocyte injury in diabetic nephropathy.

Authors - Ling Jiang
humanWB

Int J Mol Sci

PLOD2 Is a Prognostic Marker in Glioblastoma That Modulates the Immune Microenvironment and Tumor Progression.

Authors - Nina Kreße
humanWB

Int J Mol Sci

LncRNA H19-Derived miR-675-5p Accelerates the Invasion of Extravillous Trophoblast Cells by Inhibiting GATA2 and Subsequently Activating Matrix Metalloproteinases.

Authors - Manabu Ogoyama
humanWB

Cancers (Basel)

WISP2/CCN5 Suppresses Vasculogenic Mimicry through Inhibition of YAP/TAZ Signaling in Breast Cancer Cells.

Authors - Nathalie Ferrand
humanWB

Cancer Lett

Long non-coding RNA MALAT1 promotes gastric cancer tumorigenicity and metastasis by regulating vasculogenic mimicry and angiogenesis.

Authors - Yue Li