MMP23B Polyklonaler Antikörper

MMP23B Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

Konjugation

Unkonjugiert

Kat-Nr. : 13020-1-PBS

Synonyme

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• 

  WB analysis of A549 using 13020-1-AP (same clone as 13020-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 13020-1-AP (MMP23B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13020-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse liver using 13020-1-AP (same clone as 13020-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 13020-1-AP (MMP23B Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13020-1-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-3 using 13020-1-AP (same clone as 13020-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 13020-1-AP (MMP23B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13020-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 13020-1-AP (same clone as 13020-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13020-1-AP (MMP23B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13020-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 using 13020-1-AP (same clone as 13020-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 13020-1-AP (MMP23B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13020-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 13020-1-AP (same clone as 13020-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13020-1-AP (MMP23B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13020-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 13020-1-AP (same clone as 13020-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 13020-1-AP (MMP23B Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13020-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 13020-1-AP (same clone as 13020-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 13020-1-AP (MMP23B Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13020-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 13020-1-AP (same clone as 13020-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13020-1-AP (MMP23B Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13020-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 13020-1-AP (same clone as 13020-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13020-1-AP (MMP23B Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13020-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 13020-1-AP (same clone as 13020-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MMP23B antibody (13020-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 13020-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HepG2 using 13020-1-AP (same clone as 13020-1-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug MMP23B Polyclonal antibody (13020-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 13020-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

13020-1-PBS bindet in MMP23B und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MMP23B fusion protein Ag4113
• Vollständiger Name: matrix metallopeptidase 23B
• Berechnetes Molekulargewicht: 390 aa, 44 kDa
• Beobachtetes Molekulargewicht: 46-60 kDa
• GenBank-Zugangsnummer: BC025719
• Gene symbol: MMP23B
• Gene ID (NCBI): 8510
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MMP23B(matrix metallopeptidase 23B) is also named as MMP21, MMP22.It is a regulator in matrix remodeling and may be playing a role in enchondral bone formation and in regulation of cranial suture closure .It has 3 isoforms produced by alternative splicing with the molecular weight of 44KDa,48KDa and 46KDa,respectively.It also can be N-glycosylated.