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MMP25 Polyklonaler Antikörper

MMP25 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 30655-1-PBS

Synonyme

MTMMP6, MT6MMP, MT MMP6, MMP 25, matrix metallopeptidase 25

Filter:
  • Western Blot (WB) analysis of various lysates using MMP25 Polyclonal antibody (30655-1-AP)

    WB analysis using 30655-1-AP (same clone as 30655-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30655-1-AP (MMP25 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30655-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using MMP25 Polyclonal antibody (30655-1-AP)

    IHC staining of mouse spleen using 30655-1-AP (same clone as 30655-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 30655-1-AP (MMP25 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30655-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using MMP25 Polyclonal antibody (30655-1-AP)

    IF Staining of A549 using 30655-1-AP (same clone as 30655-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using MMP25 antibody (30655-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 30655-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

30655-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA MMP25 und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen MMP25 fusion protein Ag29304
Vollständiger Name matrix metallopeptidase 25
Berechnetes Molekulargewicht 63 kDa
Beobachtetes Molekulargewicht38-40 kDa
GenBank-ZugangsnummerNM_022468
Gene symbol MMP25
Gene ID (NCBI) 64386
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MMP25, also known as MT6-MMP, is one of the two glycosylphosphatidylinositol anchored matrix metalloproteinases (MMPs) that have been suggested to play a role in pericellular proteolysis. MMP25 was originally cloned from human leukocytes and its mRNA is predominantly expressed in leukocytes (polymorphonuclear cells and monocytes) of normal tissues and in brain, colon, urothelial and prostate cancers (PMID: 17513868). MMP-25 can exaggerate inflammatory responses by inactivating the main antiproteinase, a1-proteinase inhibitor or serpin (PMID: 16584348). The band at 57-kDa we detected is likely to be active MT6-MMP and minor MT6-MMP degradation product of 38-kDa.