MMP8 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

MMP8 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(15)

Kat-Nr. : 17874-1-AP

Synonyme

CLG1, EC:3.4.24.34, HNC, Matrix metalloproteinase 8, Matrix metalloproteinase-8

---

• 

  WB analysis of HepG2 using 17874-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17874-1-AP (MMP8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 17874-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 17874-1-AP (MMP8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 17874-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17874-1-AP (MMP8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse kidney using 17874-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17874-1-AP (MMP8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 17874-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 17874-1-AP (MMP8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 17874-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17874-1-AP (MMP8 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human placenta using 17874-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17874-1-AP (MMP8 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 17874-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17874-1-AP (MMP8 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 17874-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17874-1-AP (MMP8 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 17874-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17874-1-AP (MMP8 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 17874-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17874-1-AP (MMP8 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary using 17874-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17874-1-AP (MMP8 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 17874-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17874-1-AP (MMP8 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 17874-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17874-1-AP (MMP8 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 17874-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17874-1-AP (MMP8 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HepG2 using 17874-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MMP8 antibody (17874-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), (red).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, Jurkat-Zellen, Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe, humanes Nierengewebe, humanes Eierstockgewebe, humanes Milzgewebe, humanes Hodengewebe, Mausnierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2400
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 11 publications below
• IHC: See 2 publications below
• IF: See 4 publications below

Produktinformation

17874-1-AP bindet in WB, IHC, IF/ICC, ELISA MMP8 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MMP8 fusion protein Ag12367
• Vollständiger Name: matrix metallopeptidase 8 (neutrophil collagenase)
• Berechnetes Molekulargewicht: 467 aa, 53 kDa
• Beobachtetes Molekulargewicht: 60-70 kDa
• GenBank-Zugangsnummer: BC074988
• Gene symbol: MMP8
• Gene ID (NCBI): 4317
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MMP8(Matrix metalloproteinase-8) an enzyme that degrades fibrillar collagens imparting strength to the fetal membranes, is expressed by leukocytes and chorionic cytotrophoblast cells(PMID:15367487). Purified human neutrophil MMP-8 with proeMMP-8 at 70-75 KDa and active enzyme at 65 KDa and amniochorion proenzyme and active enzyme at 65 and 50 KDa, respectively(PMID: 28772283, PMID:15042023).

Publikationen

Species	Application	Title
mouse	WB	PLoS Pathog; Circumventing Y. pestis Virulence by Early Recruitment of Neutrophils to the Lungs during Pneumonic Plague.; Authors - Yaron Vagima
mouse,human	WB,IHC	Stem Cell Res Ther; Transplantation of human amniotic epithelial cells promotes morphological and functional regeneration in a rat uterine scar model.; Authors - Yihui Fan
human	WB,IHC	Am J Pathol; Preeclampsia is associated with alterations in DNA methylation of genes involved in collagen metabolism.; Authors - Mousa Ahmad A AA
mouse	IF	Viruses; Matrix Metalloproteinases Expression Is Associated with SARS-CoV-2-Induced Lung Pathology and Extracellular-Matrix Remodeling in K18-hACE2 Mice.; Authors - Hila Gutman
human	WB	Mediators Inflamm; Abnormal Expression of DICER1 Leads to Dysregulation of Inflammatory Effectors in Human Synoviocytes.; Authors - Congshan Jiang
rat	WB	Exp Eye Res; Luteolin ameliorates cornea stromal collagen degradation and inflammatory damage in rats with corneal alkali burn; Authors - Heng Wang