MMP-9 (Middle) Polyklonaler Antikörper

MMP-9 (Middle) Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(68)

Kat-Nr. : 27306-1-AP

Synonyme

MMP9 (Middle), MMP9, 67 kDa matrix metalloproteinase-9, 82 kDa matrix metalloproteinase-9, CLG4B



Geprüfte Anwendungen

Erfolgreiche Detektion in WBhumanes Speichelgewebe
Erfolgreiche IPhumanes Speichelgewebe
Erfolgreiche Detektion in IHChumanes Tonsillitisgewebe, humanes Lungenkarzinomgewebe, humanes Milzgewebe, Mauslebergewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:1000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

27306-1-AP bindet in WB, IHC, IF, IP, CoIP, ELISA MMP-9 (Middle) und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
In Publikationen genannte Reaktivitäthuman, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen MMP-9 (Middle) fusion protein Ag26132
Vollständiger Name matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)
Berechnetes Molekulargewicht 707 aa, 78 kDa
Beobachtetes Molekulargewicht 92 kDa
GenBank-ZugangsnummerBC006093
Gene symbol MMP-9
Gene ID (NCBI) 4318
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, tissue remodeling, and disease processes, such as arthritis or metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. Matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase) (MMP9, synonyms: GELB, CLG4B) degrades collagens type IV and V. Studies in rhesus monkeys suggest that MMP9 is involved in IL-8-induced mobilization hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. The pro-MMP9 is 92 kDa, and it can be detected a processed form of 68 kDa or 82 kDa. This protein can exist as a dimer of 180 kDa (PMID:7492685).

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for MMP-9 (Middle) antibody 27306-1-APProtokoll herunterladen
IHC protocol for MMP-9 (Middle) antibody 27306-1-APProtokoll herunterladenl
IP protocol for MMP-9 (Middle) antibody 27306-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
mouseIF

Adv Sci (Weinh)

Photothermal-Responsive Soluble Microneedle Patches for Meibomian Gland Dysfunction Therapy

Authors - Fei Yu
mouseIHC

Bioeng Transl Med

Macrophages overexpressing interleukin-10 target and prevent atherosclerosis: Regression of plaque formation and reduction in necrotic core

Authors - Mingyi Wang
humanWB,IHC

Int J Mol Sci

Comprehensive Analysis of MICALL2 Reveals Its Potential Roles in EGFR Stabilization and Ovarian Cancer Cell Invasion

Authors - Tianxiang Xia
mouseWB

Front Endocrinol (Lausanne)

Nicotinamide mononucleotide attenuates HIF-1α activation and fibrosis in hypoxic adipose tissue via NAD+/SIRT1 axis

Authors - Keke Wu
humanWB

Int J Biol Sci

TDG suppresses the migration and invasion of human colon cancer cells via the DNMT3A/TIMP2 axis.

Authors - Jiyu Miao
humanWB

Front Mol Biosci

Comprehensive Characterization of Ageing-Relevant Subtypes Associated With Different Tumorigenesis and Tumor Microenvironment in Prostate Cancer.

Authors - Liang Huang