MMP-9 (Middle) Polyklonaler Antikörper
MMP-9 (Middle) Polyklonal Antikörper für WB, IHC, IP, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus und mehr (1)
Anwendung
WB, IHC, IF, IP, CoIP, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 27306-1-AP
Synonyme
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | humanes Speichelgewebe |
Erfolgreiche IP | humanes Speichelgewebe |
Erfolgreiche Detektion in IHC | humanes Tonsillitisgewebe, humanes Lungenkarzinomgewebe, humanes Milzgewebe, Mauslebergewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
Empfohlene Verdünnung
Anwendung | Verdünnung |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunhistochemie (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
WB | See 62 publications below |
IHC | See 10 publications below |
IF | See 3 publications below |
CoIP | See 1 publications below |
Produktinformation
27306-1-AP bindet in WB, IHC, IF, IP, CoIP, ELISA MMP-9 (Middle) und zeigt Reaktivität mit human, Maus
Getestete Reaktivität | human, Maus |
In Publikationen genannte Reaktivität | human, Maus, Ratte |
Wirt / Isotyp | Kaninchen / IgG |
Klonalität | Polyklonal |
Typ | Antikörper |
Immunogen | MMP-9 (Middle) fusion protein Ag26132 |
Vollständiger Name | matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase) |
Berechnetes Molekulargewicht | 707 aa, 78 kDa |
Beobachtetes Molekulargewicht | 92 kDa |
GenBank-Zugangsnummer | BC006093 |
Gene symbol | MMP-9 |
Gene ID (NCBI) | 4318 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Antigen-Affinitätsreinigung |
Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, tissue remodeling, and disease processes, such as arthritis or metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. Matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase) (MMP9, synonyms: GELB, CLG4B) degrades collagens type IV and V. Studies in rhesus monkeys suggest that MMP9 is involved in IL-8-induced mobilization hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. The pro-MMP9 is 92 kDa, and it can be detected a processed form of 68 kDa or 82 kDa. This protein can exist as a dimer of 180 kDa (PMID:7492685).
Protokolle
PRODUKTSPEZIFISCHE PROTOKOLLE | |
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WB protocol for MMP-9 (Middle) antibody 27306-1-AP | Protokoll herunterladen |
IHC protocol for MMP-9 (Middle) antibody 27306-1-AP | Protokoll herunterladenl |
IP protocol for MMP-9 (Middle) antibody 27306-1-AP | Protokoll herunterladen |
STANDARD-PROTOKOLLE | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
---|---|---|
Adv Sci (Weinh) Photothermal-Responsive Soluble Microneedle Patches for Meibomian Gland Dysfunction Therapy | ||
Bioeng Transl Med Macrophages overexpressing interleukin-10 target and prevent atherosclerosis: Regression of plaque formation and reduction in necrotic core | ||
Int J Mol Sci Comprehensive Analysis of MICALL2 Reveals Its Potential Roles in EGFR Stabilization and Ovarian Cancer Cell Invasion | ||
Front Endocrinol (Lausanne) Nicotinamide mononucleotide attenuates HIF-1α activation and fibrosis in hypoxic adipose tissue via NAD+/SIRT1 axis | ||
Int J Biol Sci TDG suppresses the migration and invasion of human colon cancer cells via the DNMT3A/TIMP2 axis. | ||
Front Mol Biosci Comprehensive Characterization of Ageing-Relevant Subtypes Associated With Different Tumorigenesis and Tumor Microenvironment in Prostate Cancer. |