MMP-9 Rekombinanter Antikörper

MMP-9 Rekombinant Antikörper für Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

241224B5

Kat-Nr. : 82685-16-PBS

Synonyme

MMP9, GELB, EC:3.4.24.35, CLG4B, 82 kDa matrix metalloproteinase-9

---

• 

  Sandwich ELISA standard curve of MP01003-2

  Sandwich ELISA standard curve of MP01003-2, Human MMP-9 Recombinant Matched Antibody Pair - PBS only. 82685-18-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0442. 82685-16-PBS was HRP conjugated as the detection antibody. Range: 39-2500 pg/mL.

• 

  Sample test of MP01003-2

  Serum of sixteen individual healthy human donors was measured. The MMP-9 concentration of detected samples was determined to be 213.0 ng/mL with a range of 67.5 - 426.4 ng/mL.

• 

  Sample test of MP01003-2

  Saliva of eight individual healthy human donors was measured. The MMP-9 concentration of detected samples was determined to be 80.7 ng/mL with a range of 1.9 - 260.9 ng/mL.

• 

  Sample test of MP01003-2

  Urine of eight individual healthy human donors was measured. The MMP-9 concentration of detected samples was determined to be 207.3 pg/mL with a range of 11.4 - 841.8 pg/mL.

• 

  Sample test of MP01003-2

  Human peripheral blood mononuclear cells (PBMC) were cultured unstimulated or stimulated with 10 μg/mL PHA for 5 days. The mean MMP-9 concentration was determined to be 157.3 ng/mL in unstimulated PBMC supernatant, 237.9 ng/mL in PHA stimulated PBMC supernatant.

• 

  Cytometric bead array standard curve of MP01003-2

  Cytometric bead array standard curve of MP01003-2, MMP-9 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 82685-18-PBS. Detection antibody: 82685-16-PBS. Standard: Eg0442. Range: 0.625-80 ng/mL.

• 

  Affinity and Kinetic Characterization of 82685-16-PBS

  Biolayer interferometry (BLl) kinetic assay of 82685-16-PBS against Human MMP-9 was performed. The affinity constant is 0.726 nM.

---

Geprüfte Anwendungen

Produktinformation

82685-16-PBS bindet in Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test MMP-9 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)
• Berechnetes Molekulargewicht: 707 aa, 78 kDa
• GenBank-Zugangsnummer: BC006093
• Gene symbol: MMP-9
• Gene ID (NCBI): 4318
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.