CD206 Rekombinanter Antikörper

CD206 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

Unkonjugiert

Publikationen(3)

CloneNo.

240344D12

Kat-Nr. : 98031-1-RR

Synonyme

Mrc1, MR, MMR, mannose receptor, C type 1, 240344D12

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  FC experiment of mouse peritoneal macrophages using 98031-1-RR

  1x10^6 mouse peritoneal macrophages were surface stained with 0.25 ug Anti-Mouse MMR/CD206 Rabbit Recombinant Antibody (98031-1-RR, Clone: 240344D12) or Isotype Control and PE-conjugated Goat Anti-Rabbit IgG. Cells were then stained with APC Anti-Mouse CD107b / LAMP2. Cells were not fixed.

• 

  FC experiment of mouse peritoneal macrophages using 98031-1-RR

  1x10^6 mouse peritoneal macrophages were surface stained with 0.25 ug Anti-Mouse MMR/CD206 Rabbit Recombinant Antibody (98031-1-RR, Clone: 240344D12) (red) or Isotype Control (blue) and PE-conjugated Goat Anti-Rabbit IgG. Cells were not fixed.

• 

  Affinity and Kinetic Characterization of 98031-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98031-1-RR against Mouse CD206 were performed. The affinity constant is 75.8 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: peritoneale Makrophagen der Maus

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• FC: See 2 publications below

Produktinformation

98031-1-RR bindet in FC CD206 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: mannose receptor, C type 1
• Berechnetes Molekulargewicht: 165 kDa
• GenBank-Zugangsnummer: NM_008625.2
• Gene symbol: CD206
• Gene ID (NCBI): 17533
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Stable for one year after shipment.

Hintergrundinformationen

CD206, also named as MMR, CLEC13D and MRC1, is a type I membrane receptor that mediates the endocytosis of glycoproteins by macrophages. CD206 has been shown to bind high-mannose structures on the surface of potentially pathogenic viruses, bacteria, and fungi so that they can be neutralized by phagocytic engulfment. CD206 is a 170 kDa transmembrane protein which contains 5 domains: an amino-terminal cysteine-rich region, a fibronectin type II repeat, a series of eight tandem lectin-like carbohydrate recognition domains (responsible for the recognition of mannose and fucose), a transmembrane domain, and an intracellular carboxy-terminal tail. It is expressed on most tissue macrophages, in vitro derived dendritic cells, lymphatic and sinusoidal endothelia.

Publikationen

Species	Application	Title
mouse	FC	Inflammation; Moxibustion Alleviates Inflammation via SIRT5-mediated Post-translational Modification and Macrophage Polarization; Authors - Chuan-Yi Zuo
		Bioact Mater; A ceramic microbridge microfluidic chip to study osteogenic differentiation of mesenchymal stem cells in bioactive ceramic immune microenvironment; Authors - Sheng Ye
mouse	FC	Acta Biomater; An injectable and adaptable system for the sustained release of hydrogen sulfide for targeted diabetic wound therapy by improving the microenvironment of inflammation regulation and angiogenesis; Authors - Hao Zhang