MMS19 Monoklonaler Antikörper

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• KD/KO Validated

MMS19 Monoklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2H6D8

Kat-Nr. : 66049-1-PBS

Synonyme

hMMS19, MET18, MET18 homolog, MMS19, MMS19 like protein, MMS19L

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  WB analysis of HeLa using 66049-1-Ig (same clone as 66049-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66049-1-Ig (MMS19 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66049-1-PBS in a different storage buffer formulation.

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  WB analysis of fetal human brain using 66049-1-Ig (same clone as 66049-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66049-1-Ig (MMS19 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66049-1-PBS in a different storage buffer formulation.

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  WB analysis of rat brain using 66049-1-Ig (same clone as 66049-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66049-1-Ig (MMS19 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66049-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 66049-1-Ig (same clone as 66049-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66049-1-Ig (MMS19 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66049-1-PBS in a different storage buffer formulation.

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  WB analysis of RAW 264.7 using 66049-1-Ig (same clone as 66049-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66049-1-Ig (MMS19 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66049-1-PBS in a different storage buffer formulation.

• 

  WB analysis of RAW 264.7 using 66049-1-Ig (same clone as 66049-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66049-1-Ig (MMS19 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66049-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 66049-1-Ig (same clone as 66049-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 66049-1-Ig (MMS19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66049-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 66049-1-Ig (same clone as 66049-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66049-1-Ig (MMS19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66049-1-PBS in a different storage buffer formulation.

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  WB analysis of human kidney using 66049-1-Ig (same clone as 66049-1-PBS)

  human kidney tissue were subjected to SDS PAGE followed by western blot with 66049-1-Ig (MMS19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66049-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 66049-1-Ig (same clone as 66049-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66049-1-Ig (MMS19 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66049-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66049-1-Ig (same clone as 66049-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66049-1-Ig (MMS19 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66049-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 66049-1-Ig (same clone as 66049-1-PBS)

  IP result of anti-MMS19 (IP:66049-1-Ig, 4ug; Detection:66049-1-Ig 1:2000) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 66049-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 66049-1-Ig (same clone as 66049-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66049-1-Ig (MMS19 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66049-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 66049-1-Ig (same clone as 66049-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66049-1-Ig (MMS19 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66049-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 66049-1-Ig (same clone as 66049-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 66049-1-Ig(MMS19 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66049-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66049-1-Ig (same clone as 66049-1-PBS)

  Immunofluorescent analysis of fixed Hela cells using 66049-1-Ig (MMS19 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Mouse IgG. This data was developed using the same antibody clone with 66049-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66049-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA MMS19 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: MMS19 fusion protein Ag8960
• Vollständiger Name: MMS19 nucleotide excision repair homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 1030 aa, 113 kDa
• Beobachtetes Molekulargewicht: 103 kDa, 38 kDa
• GenBank-Zugangsnummer: BC006575
• Gene symbol: MMS19
• Gene ID (NCBI): 64210
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MMS19 (MMS19 nucleotide excision repair homolog), also known as MET18, is a 1,030 amino acid nuclear protein containing seven HEAT repeats that belongs to the MET18/MMS19 family. Via its interactions with TFIIH p80 and TFIIH p89 helicases, MMS19 plays a role in nucleotide excision repair (NER) and RNA polymerase II (Pol II) transcription. MMS19 may also function as a transcriptional coactivator of estrogen receptor. While ubiquitously expressed, highest levels of MMS19 have been found in testis. At least five distinct MMS19 protein isoforms exist, which are produced by alternative splicing events.