MN1 Polyklonaler Antikörper

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MN1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 24697-1-AP

Synonyme

MGCR, MGCR1, MGCR1 PEN, Transcriptional activator MN1

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  WB analysis using 24697-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24697-1-AP (MN1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse skeletal muscle using 24697-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 24697-1-AP (MN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 24697-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 24697-1-AP (MN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skeletal muscle using 24697-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 24697-1-AP (MN1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skeletal muscle using 24697-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 24697-1-AP (MN1 Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, U2OS-Zellen
• Erfolgreiche Detektion in IHC: Maus-Skelettmuskelgewebe, humanes Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Produktinformation

24697-1-AP bindet in WB, IHC, IF, ELISA MN1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MN1 fusion protein Ag20344
• Vollständiger Name: meningioma (disrupted in balanced translocation) 1
• Berechnetes Molekulargewicht: 1320 aa, 136 kDa
• Beobachtetes Molekulargewicht: 136 kDa
• GenBank-Zugangsnummer: BC156879
• Gene symbol: MN1
• Gene ID (NCBI): 4330
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MN1 (Transcriptional activator MN1), which is mainly located in nucleus. Highest expression is observed in fetal brain and skeletal muscle, and adult skeletal muscle. MN1 protein can interact with Brg1/Brm related factor (BAF) complex containing Smarca4/Brg1 and stabilize it on chromatin, thus maintaining the expression of hematopoietic progenitor cell-like genes. Under normal physiological conditions, MN1 protein is mainly expressed in granulocyte monocyte progenitor cells (GMP) in hematopoietic system, which plays an important role in the development and function of hematopoietic cells, and it is involved in regulating cell proliferation, differentiation, apoptosis and embryonic development. MN1 protein is related to many diseases, especially in leukemia (PMID: 23049943). MN1 gene rearrangements such as t(12; 22)(p13; Q11) can produce MN1-TEL fusion protein, which combines the transcriptional activation domain of MN1 and the DNA binding domain of TEL(ETV6), and can stably occupy the TEL recognition sequence, hindering the combination of normal transcription regulatory factors, thus leading to leukemia. Overexpression of MN1 gene has also been proved to be one of the signs of poor prognosis in patients with acute myeloid leukemia (AML), and its expression level is high in AML patients with normal karyotype. The molecular weight of MN1 is 136 kDa.

Publikationen

Species	Application	Title
mouse	IHC,IF	Nat Commun; Astroblastomas exhibit radial glia stem cell lineages and differential expression of imprinted and X-inactivation escape genes.; Authors - Norman L Lehman
human	WB,IHC	EBioMedicine; METTL14-mediated epitranscriptome modification of MN1 mRNA promote tumorigenicity and all-trans-retinoic acid resistance in osteosarcoma.; Authors - Hong-Bo Li ; KD Validated
human	IHC	Acta Neuropathol; MN1 immunohistochemistry is a sensitive diagnostic biomarker for primitive CNS tumors with MN1 fusion; Authors - Roxane Daniel
human	WB	J Transl Med; Modulation of DAPK1 expression by its alternative splice variant DAPK1-215 in cancer; Authors - QingShui Wang