MNAT1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

MNAT1 Polyklonal Antikörper für IF, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 11719-1-AP

Synonyme

CAP35, CDK7/cyclin H assembly factor, Cyclin G1 interacting protein, MAT1, Menage a trois, MNAT1, p35, p36, RING finger protein 66, RING finger protein MAT1, RNF66, TFB3

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Galerie der Validierungsdaten

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  WB analysis using 11719-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11719-1-AP (MNAT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 11719-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 11719-1-AP (MNAT1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 11719-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 11719-1-AP (MNAT1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 11719-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 11719-1-AP (MNAT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 11719-1-AP

  IP Result of anti-MNAT1 (IP:11719-1-AP, 4ug; Detection:11719-1-AP 1:500) with HeLa cells lysate 1200ug.

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  IF Staining of A375 using 11719-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A375 cells using MNAT1 antibody (11719-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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  IF Staining of HeLa using 11719-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MNAT1 antibody (11719-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A375-Zellen, A431-Zellen, HeLa-Zellen, MCF-7-Zellen, NIH/3T3-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IF: A375-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 2 publications below

Produktinformation

11719-1-AP bindet in WB, IP, IHC, IF, ELISA MNAT1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MNAT1 fusion protein Ag2293
• Vollständiger Name: menage a trois homolog 1, cyclin H assembly factor (Xenopus laevis)
• Berechnetes Molekulargewicht: 309 aa, 36 kDa
• Beobachtetes Molekulargewicht: 36 kDa
• GenBank-Zugangsnummer: BC000820
• Gene symbol: MNAT1
• Gene ID (NCBI): 4331
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	IHC	Int J Biol Sci; Therapeutic Targeting of CDK7 Suppresses Tumor Progression in Intrahepatic Cholangiocarcinoma.; Authors - Hua-Dong Chen
human	WB,IHC	BMC Cancer; MNAT1 promotes proliferation and the chemo-resistance of osteosarcoma cell to cisplatin through regulating PI3K/Akt/mTOR pathway.; Authors - Chensheng Qiu ; KD Validated
mouse	WB	Acta Histochem; Cyclin-dependent kinase 5 regulates MAPK/ERK signaling in the skin of mice.; Authors - Xuexian Liu
mouse	WB	Genes Dev; TRF1 uses a noncanonical function of TFIIH to promote telomere replication; Authors - Zhe Yang