MNK1 Polyklonaler Antikörper

MNK1 Polyklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10136-1-PBS

Synonyme

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  WB analysis of HeLa using 10136-1-AP (same clone as 10136-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 10136-1-AP (MNK1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10136-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HL-60 using 10136-1-AP (same clone as 10136-1-PBS)

  HL-60 cells were subjected to SDS PAGE followed by western blot with 10136-1-AP (MNK1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10136-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 10136-1-AP (same clone as 10136-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10136-1-AP (MNK1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10136-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 10136-1-AP (same clone as 10136-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10136-1-AP (MNK1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10136-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse small intestine using 10136-1-AP (same clone as 10136-1-PBS)

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 10136-1-AP (MNK1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10136-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 10136-1-AP (same clone as 10136-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MNK1 antibody (10136-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10136-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

10136-1-PBS bindet in WB, IF/ICC, Indirect ELISA MNK1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MNK1 fusion protein Ag0184
• Vollständiger Name: MAP kinase interacting serine/threonine kinase 1
• Berechnetes Molekulargewicht: 51 kDa
• Beobachtetes Molekulargewicht: 51 kDa, 47 kDa
• GenBank-Zugangsnummer: BC002755
• Gene symbol: MNK1
• Gene ID (NCBI): 8569
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MNK1 (mitogen-activated protein kinase-interacting kinase 1) was discovered as a consequence of bacterial expression libraries screening for proteins regulated by the classical mitogen-activated protein kinases, the ERKs (extracellular signal-regulated kinases). Phosphorylation of Mnk1 has been shown to activate its kinase activity as well as to enhance its binding to the eukaryotic initiation factor 4G (eIF4G) which functions as a scaffolding protein. Human MNK1 arises from an alternatively spliced transcript giving rise to 2 isoforms, longer MNK1a (50 kDa) and a shorter MNK1b variant (38 kDa) that lacks 89 C-terminal amino acids(PMID: 21406405).

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