MNT Rekombinanter Antikörper

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• KD/KO Validated

MNT Rekombinant Antikörper für WB, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte

Anwendung

WB, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

240550A10

Kat-Nr. : 83648-1-PBS

Synonyme

240550A10, bHLHd3, Class D basic helix-loop-helix protein 3, MAD6, MAX binding protein

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  WB analysis using 83648-1-RR (same clone as 83648-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83648-1-RR (MNT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83648-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 83648-1-RR (same clone as 83648-1-PBS)

  WB result of MNT antibody (83648-1-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MNT transfected HeLa cells. This data was developed using the same antibody clone with 83648-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP00617-1

  Sandwich ELISA standard curve of MP00617-1, Human MNT Recombinant Matched Antibody Pair - PBS only. 83648-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag34529. 83648-3-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL.

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  Sample test of MP00617-1

  The mean MNT concentration was determined to be 19.62 ng/mL in HEK-293T cell extract based on a 5.50 mg/mL extract load and 17.06 ng/mL in HeLa cell extract based on a 3.60 mg/mL extract load.

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  IF Staining of HeLa using 83648-1-RR (same clone as 83648-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MNT antibody (83648-1-RR, Clone: 240550A10 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83648-1-PBS in a different storage buffer formulation.

• 

  FC experiment of A431 using 83648-1-RR (same clone as 83648-1-PBS)

  1x10^6 A431 cells were intracellularly stained with 0.25 ug MNT Recombinant antibody (83648-1-RR, Clone:240550A10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83648-1-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP00617-1

  Cytometric bead array standard curve of MP00617-1, MNT Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83648-1-PBS. Detection antibody: 83648-3-PBS. Standard: Ag34529. Range: 0.625-80 ng/mL.

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  Affinity and Kinetic Characterization of 83648-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83648-1-RR against Human MNT were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

83648-1-PBS bindet in WB, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test MNT und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: MNT fusion protein Ag34529
• Vollständiger Name: MAX binding protein
• Berechnetes Molekulargewicht: 582 aa, 62 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC117563
• Gene symbol: MNT
• Gene ID (NCBI): 4335
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Mnt (Max binding protein), also known as MAD6, ROX, bHLHd3 (class D basic helix-loop-helix protein 3) or MXD6, is a 582 amino acid nuclear protein that forms a complex with Max (Myc-associated factor X) to repress transcription. Mnt contains one basic helix-loop-helix (bHLH) domain and is encoded by a gene that maps to human chromosome 17p13.3. Chromosome 17 comprises over 2.5% of the human genome and encodes over 1,200 genes. Two key tumor suppressor genes are associated with chromosome 17, namely, p53 and BRCA1. Tumor suppressor p53 is necessary for maintenance of cellular genetic integrity by moderating cell fate through DNA repair versus cell death. Like p53, BRCA1 is directly involved in DNA repair, though specifically it is recognized as a genetic determinant of early onset breast cancer and predisposition to cancers of the ovary, colon, prostate gland and fallopian tubes.