MORF4L1 Polyklonaler Antikörper

MORF4L1 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 55257-1-AP

Synonyme

Eaf3, FWP006, HSPC008, HSPC061, HsT17725, MORF related gene 15 protein, MORF4L1, MORFRG15, mortality factor 4 like 1, MRG15, PP368, Protein MSL3 1, S863 6

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  WB analysis of HEK-293 using 55257-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 55257-1-AP (MORF4L1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 55257-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 55257-1-AP (MORF4L1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 55257-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55257-1-AP (MORF4L1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 55257-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 55257-1-AP (MORF4L1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 55257-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55257-1-AP (MORF4L1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 55257-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 55257-1-AP (MORF4L1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 55257-1-AP

  IP result of anti-MORF4L1 (IP:55257-1-AP, 4ug; Detection:55257-1-AP 1:800) with HEK-293 cells lysate 1200ug.

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  IHC staining of human skin using 55257-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 55257-1-AP (MORF4L1 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human testis using 55257-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 55257-1-AP (MORF4L1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 55257-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 55257-1-AP (MORF4L1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 55257-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 55257-1-AP (MORF4L1 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human testis using 55257-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 55257-1-AP (MORF4L1 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human testis using 55257-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 55257-1-AP (MORF4L1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 55257-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 55257-1-AP (MORF4L1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skin using 55257-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 55257-1-AP (MORF4L1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skin using 55257-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 55257-1-AP (MORF4L1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skin using 55257-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 55257-1-AP (MORF4L1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human skin using 55257-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 55257-1-AP (MORF4L1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human skin using 55257-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 55257-1-AP (MORF4L1 antibody) at dilution of 1:200 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, A549-Zellen, Maushirngewebe
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Hautgewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

55257-1-AP bindet in WB, IHC, IP, ELISA MORF4L1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: mortality factor 4 like 1
• Berechnetes Molekulargewicht: 41 kDa
• Beobachtetes Molekulargewicht: 35-37 kDa
• GenBank-Zugangsnummer: NM_006791
• Gene symbol: MORF4L1
• Gene ID (NCBI): 10933
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MORF4L1, also named as MRG15, belongs to the MRG family. It is a component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. It is also component of the mSin3A complex which acts to repress transcription by deacetylation of nucleosomal histones. (PMID:14966270) This antibody has no cross-reaction to MORF4L2.

Publikationen

Species	Application	Title
human	WB	bioRxiv; Dynamic Regulation OF The Chromatin Environment By Ash1L Modulates Human Neuronal Structure And Function; Authors - Megha Jhanji